Mast Cells Exhibiting Strong Cytoplasmic Staining for IgE and High Affinity IgE Receptor are Increased in IgG4-Related Disease.
Mast Cells Exhibiting Strong Cytoplasmic Staining for IgE and High Affinity IgE Receptor are Increased in IgG4-Related Disease.
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DOI：
10.1038/s41598-018-23043-9
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发表时间：
2018-03-15
影响因子：
4.6
通讯作者：
Sato Y
中科院分区：
文献类型：
--
作者：
Nishida K;Gion Y;Takeuchi M;Tanaka T;Kataoka TR;Yoshino T;Sato Y
免疫球蛋白G4（IgG 4）相关疾病的特征是血清IgG 4水平升高和IgG 4阳性细胞数量增加。然而，其发病机制尚未完全了解。我们以前认为肥大细胞可能在IgG 4相关疾病中发挥重要作用。在这项研究中，我们证实了肥大细胞在IgG 4相关的淋巴结病的特点，通过使用免疫组化和双重免疫荧光。我们分析了23例IgG 4相关淋巴结病，并与23例非特异性淋巴样增生进行了比较。大多数IgG 4相关淋巴结病患者的宫颈病变累及其他器官。免疫组织学上，IgG 4相关淋巴结病中免疫球蛋白E和高亲和力免疫球蛋白E受体胞质染色较强的肥大细胞显著增加，与非特异性淋巴样增生相比（平均值：每个高倍视野3.83 ± 3.99个细胞和7.14 ± 8.21个细胞; P = 0.007和P = 0.011）。此外，双重免疫荧光试验显示，免疫球蛋白E和高亲和力免疫球蛋白E受体染色在IgG 4相关淋巴结病中表现出细胞质颗粒图案，表明抗体和受体的内化。我们的研究结果表明，肥大细胞活化可能参与了IgG 4相关疾病的发病机制。
Immunoglobulin G4 (IgG4)-related disease is characterized by elevated serum IgG4 levels and increased numbers of IgG4-positive cells. However, its pathogenesis is not fully understood. We previously suggested that mast cells may play an important role in IgG4-related disease. In this study, we confirmed the characteristics of mast cells in IgG4-related lymphadenopathy by using immunohistochemistry and dual immunofluorescence. We analyzed 23 cases of IgG4-related lymphadenopathy and compared them with 23 cases of non-specific lymphoid hyperplasia. The majority of patients with IgG4-related lymphadenopathy had cervical lesions with involvement of other organs. Immunohistologically, mast cells with strong cytoplasmic staining for immunoglobulin E and high affinity immunoglobulin E receptor were significantly increased in IgG4-related lymphadenopathy as compared to those in non-specific lymphoid hyperplasia (mean: 3.83 ± 3.99 cells per high power field and 7.14 ± 8.21 cells per high power field, respectively; P = 0.007 and P = 0.011). In addition, dual immunofluorescence assay showed that immunoglobulin E and high affinity immunoglobulin E receptor staining exhibited a cytoplasmic granular pattern in IgG4-related lymphadenopathy, suggesting internalization of the antibodies and receptors. Our findings showed that mast cell activation might be involved in the pathogenesis of IgG4-related disease.
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影响因子：
4.9
作者：
Tsuboi H;Matsuo N;Iizuka M;Tsuzuki S;Kondo Y;Tanaka A;Moriyama M;Matsumoto I;Nakamura S;Sumida T
通讯作者：
Sumida T
影响因子：
27.4
作者：
Wallace ZS;Mattoo H;Carruthers M;Mahajan VS;Della Torre E;Lee H;Kulikova M;Deshpande V;Pillai S;Stone JH
通讯作者：
Stone JH
DOI：
10.1038/nri2782
发表时间：
2010-06
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
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影响因子：
158.5
作者：
Hamano, H;Kawa, S;Kiyosawa, K
通讯作者：
Kiyosawa, K
影响因子：
4.9
作者：
Akiyama M;Yasuoka H;Yamaoka K;Suzuki K;Kaneko Y;Kondo H;Kassai Y;Koga K;Miyazaki T;Morita R;Yoshimura A;Takeuchi T
通讯作者：
Takeuchi T