The recombination activation gene 1 (Rag1) is expressed in a subset of zebrafish olfactory neurons but is not essential for axon targeting or amino acid detection.
The recombination activation gene 1 (Rag1) is expressed in a subset of zebrafish olfactory neurons but is not essential for axon targeting or amino acid detection.
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DOI：
10.1186/1471-2202-6-46
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发表时间：
2005-07-15
期刊：
影响因子：
2.4
通讯作者：
Jesuthasan S
中科院分区：
文献类型：
--
作者：
Feng B;Bulchand S;Yaksi E;Friedrich RW;Jesuthasan S
Rag 1（转录激活基因-1）介导基因组重排，是脊椎动物获得性免疫所必需的。该基因也在嗅上皮中表达，但其功能尚不清楚。使用转基因斑马鱼线和免疫荧光，我们表明，Rag 1的表达和翻译的嗅觉感觉神经元（OSNs）的一个子集。在Rag 1启动子下表达GFP的神经元仅将其轴突投射到嗅球的外侧区域，并且具有最高水平GFP的轴突终止于单个肾小球结构中。GFP表达神经元的一个子集含有Gαo，这是微绒毛神经元的标志物。GFP阳性神经元均不表达Gαolf、Gαq和嗅觉标记蛋白OMP。耗尽RAG 1，吗啉介导的敲低或突变，不影响轴突靶向。钙成像表明，氨基酸引起Rag 1突变体的化学定位组织肾小球活动模式。Rag 1表达仅限于斑马鱼嗅神经元投射到外侧嗅球的亚群。RAG 1催化活性对于轴突靶向不是必需的，也不可能是调节气味受体表达或OSN对氨基酸的响应所必需的。
Rag1 (Recombination activation gene-1) mediates genomic rearrangement and is essential for adaptive immunity in vertebrates. This gene is also expressed in the olfactory epithelium, but its function there is unknown. Using a transgenic zebrafish line and immunofluorescence, we show that Rag1 is expressed and translated in a subset of olfactory sensory neurons (OSNs). Neurons expressing GFP under the Rag1 promoter project their axons to the lateral region of the olfactory bulb only, and axons with the highest levels of GFP terminate in a single glomerular structure. A subset of GFP-expressing neurons contain Gαo, a marker for microvillous neurons. None of the GFP-positive neurons express Gαolf, Gαq or the olfactory marker protein OMP. Depletion of RAG1, by morpholino-mediated knockdown or mutation, did not affect axon targeting. Calcium imaging indicates that amino acids evoke chemotopically organized glomerular activity patterns in a Rag1 mutant. Rag1 expression is restricted to a subpopulation of zebrafish olfactory neurons projecting to the lateral olfactory bulb. RAG1 catalytic activity is not essential for axon targeting, nor is it likely to be required for regulation of odorant receptor expression or the response of OSNs to amino acids.
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