Promotion of quality standard of herbal medicine by constituent removing and adding.
Promotion of quality standard of herbal medicine by constituent removing and adding.
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通过成分去除和添加提高中药质量标准
DOI：
10.1038/srep03668
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发表时间：
2014-01-13
影响因子：
4.6
通讯作者：
Xiao X
中科院分区：
文献类型：
--
作者：
Yan D;Li J;Xiong Y;Zhang C;Luo J;Han Y;Wang R;Jin C;Qian H;Li J;Qiu L;Peng C;Lin Y;Song X;Xiao X
为了鉴定黄连中的主要活性成分并测定其含量，我们借鉴了功能遗传学的方法，运用了去加法的概念。由于该草药具有抑菌作用，并用于治疗细菌性腹泻，我们采用微量热法检测了小檗碱、巴马汀、黄连碱、表小檗碱、黄连素和柱状胺等单一成分对志贺氏菌生长的影响。去除和添加过程表明，黄连的主要抑菌成分为黄连素和黄连碱，其抑菌率分别为54.10%和39.75%。黄连中黄连素的相对含量为8.08%~31.92%，黄连中黄连碱的相对含量为4.05%~14.45%。在整体效应的基础上，采用加减法和生物测定法相结合的方法对中草药中的有效成分进行鉴定和含量测定，可为其他天然产物的研究提供参考。
To identify major active constituents and measure their levels in a typical medicinal herb–Rhizoma coptidis, we applied the concept of removing and adding, taking inspiration from functional genetic methods. As this herb has bacteriostatic properties and is used to treat bacterial diarrhea, we examined the effects of individual constituents (berberine, palmatine, coptisine, epiberberine, jateorrhizine and columbamine) on the growth of Shigella dysenteriae with microcalorimetry. The removing and adding procedures revealed that berberine and coptisine were the main antibacterial constituents of R. coptidis, with bacteriostatic activities of 54.10% and 39.75%, respectively. The relative levels of berberine and coptisine in R. coptidis were 8.08%–31.92% and 4.05%–14.45%, respectively. On the basis of whole effect, the method of constituents removing and adding, coupled with a bioassay, is a useful strategy to identify the active constituents and measure their levels in herbal medicines, which may provide reference to other natural products.
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影响因子：
7.4
作者：
Bruce, Stephen J.;Tavazzi, Isabelle;Guy, Philippe A.
通讯作者：
Guy, Philippe A.
DOI：
10.1016/j.jpba.2009.09.006
发表时间：
2010-02-05
影响因子：
3.4
作者：
Inbaraj, B. Stephen;Lu, H.;Chen, B. H.
通讯作者：
Chen, B. H.
加速溶剂萃取结合光电二极管阵列超高效液相色谱分析和串联质谱检测分析黄连中的生物碱
影响因子：
6.2
作者：
Chen, Junhui;Wang, Fenymei;Yang, Huanghao
通讯作者：
Yang, Huanghao
影响因子：
11.4
作者：
MCGUINNESS, SM;BARISAS, BG
通讯作者：
BARISAS, BG
使用高分辨率的质谱和质谱滤波来分析合成大麻素，对设计师药物的非目标筛查的影响。
影响因子：
7.4
作者：
Grabenauer M;Krol WL;Wiley JL;Thomas BF
通讯作者：
Thomas BF