Osteoarticular tissue infection and development of skeletal pathology in murine brucellosis.
Osteoarticular tissue infection and development of skeletal pathology in murine brucellosis.
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DOI：
10.1242/dmm.011056
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发表时间：
2013-05
影响因子：
4.3
通讯作者：
Splitter GA
中科院分区：
文献类型：
--
作者：
Magnani DM;Lyons ET;Forde TS;Shekhani MT;Adarichev VA;Splitter GA
布鲁氏菌病是一种常见的细菌性人畜共患病，可引起多器官受累的慢性疾病。虽然急性布鲁氏菌病是很好的研究使用鼠动物模型，宿主-病原体相互作用的长期并发症在很大程度上仍然难以捉摸。人类布鲁氏菌病常导致持续性慢性骨关节系统受累，并伴有关节炎、脊柱炎和骶髂关节炎等并发症。在这里，我们专注于确定小鼠中的感染部位，平行于在患者中观察到的羊种布鲁氏菌病灶。体内成像显示细菌快速扩散到小鼠中轴骨骼的多个部位。与这些发现一致，免疫组织化学显示细菌存在于骨骼和四肢中，以及中轴骨骼的下脊椎骨中，它们优先位于骨髓中。令人惊讶的是，一些动物在感染后在爪子和脊柱中出现关节炎，但在这些部位没有明显的细菌。小鼠骨骼中布鲁氏菌的鉴定证实了人类的发现，即这些骨关节部位是布鲁氏菌在宿主中持续存在的重要壁龛，但介导这些部位病理表现的机制仍不清楚。未来研究骨关节组织病灶内的免疫反应可能阐明重要的组织损伤介质和布鲁氏菌生存策略。
Brucellosis, a frequent bacterial zoonosis, can produce debilitating chronic disease with involvement of multiple organs in human patients. Whereas acute brucellosis is well studied using the murine animal model, long-term complications of host-pathogen interaction remain largely elusive. Human brucellosis frequently results in persistent, chronic osteoarticular system involvement, with complications such as arthritis, spondylitis and sacroiliitis. Here, we focused on identifying infectious sites in the mouse that parallel Brucella melitensis foci observed in patients. In vivo imaging showed rapid bacterial dispersal to multiple sites of the murine axial skeleton. In agreement with these findings, immunohistochemistry revealed the presence of bacteria in bones and limbs, and in the lower spine vertebrae of the axial skeleton where they were preferentially located in the bone marrow. Surprisingly, some animals developed arthritis in paws and spine after infection, but without obvious bacteria in these sites. The identification of Brucella in the bones of mice corroborates the findings in humans that these osteoarticular sites are important niches for the persistence of Brucella in the host, but the mechanisms that mediate pathological manifestations in these sites remain unclear. Future studies addressing the immune responses within osteoarticular tissue foci could elucidate important tissue injury mediators and Brucella survival strategies.
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影响因子：
3.7
作者：
Durward M;Radhakrishnan G;Harms J;Bareiss C;Magnani D;Splitter GA
通讯作者：
Splitter GA
影响因子：
3.6
作者：
Contag, CH;Contag, PR;Benaron, DA
通讯作者：
Benaron, DA
影响因子：
1.8
作者：
ENRIGHT, FM;ARAYA, LN;WINTER, AJ
通讯作者：
WINTER, AJ
影响因子：
--
作者：
Silva TM;Costa EA;Paixão TA;Tsolis RM;Santos RL
通讯作者：
Santos RL
影响因子：
8.3
作者：
Ito, D;Tanaka, K;Fukuuchi, Y
通讯作者：
Fukuuchi, Y