Comparative analysis of C-type lectin domain proteins in the ghost moth, Thitarodes xiaojinensis (Lepidoptera: Hepialidae).
Comparative analysis of C-type lectin domain proteins in the ghost moth, Thitarodes xiaojinensis (Lepidoptera: Hepialidae).
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小金蛾Thitarodes xiaojinensis（鳞翅目：蝙蝠蛾科）C型凝集素结构域蛋白的比较分析
DOI：
10.1111/1744-7917.12564
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发表时间：
2019-06
期刊：
影响因子：
4
通讯作者：
Zou Z
中科院分区：
文献类型：
--
作者：
Meng Q;Zhang JH;Zhang H;Zhou GL;Ni RY;Zhao YN;Qin QL;Zou Z
昆虫有一个大的C型凝集素家族，参与细胞粘附、病原体识别和免疫反应的激活。在本研究中，从小金氏拟南芥转录组中鉴定出32个编码C型凝集素结构域蛋白（ctldp）的转录本。根据结构域结构，6个具有一个碳水化合物识别结构域（CRD）的ctldp被划分为CTL - S亚家族。另外23个具有2个crd的ctldp被归为免疫素（IML）亚家族。其余三个具有额外调控结构域的被分类为CTL - X亚家族。系统发育分析表明，来自不同昆虫的CTL - S和CTL - X成员可以形成同源基团。而在其他昆虫中未发现小金蠓的IML同源物。在这个亚家族中观察到显著的谱系特异性扩展，这反映了这些ctldp作为重要的受体，已经进化出多样化的成员来响应各种微生物。结合配体的预测显示，T. xiaojinensis是一种适应寒冷的物种，保留了CRDs与Ca2+结合的能力，以防止其受体冻结。不同免疫刺激后CTLDP基因诱导的比较分析表明，iml可能在免疫防御中发挥重要作用。本研究为进一步研究小金柽柳CTLDPs提供了依据。
Insects have a large family of C‐type lectins involved in cell adhesion, pathogen recognition and activation of immune responses. In this study, 32 transcripts encoding C‐type lectin domain proteins (CTLDPs) were identified from the Thitarodes xiaojinensis transcriptome. According to their domain structures, six CTLDPs with one carbohydrate‐recognition domain (CRD) were classified into the CTL‐S subfamily. The other 23 CTLDPs with two CRDs were grouped into the immulectin (IML) subfamily. The remaining three with extra regulatory domains were sorted into the CTL‐X subfamily. Phylogenetic analysis showed that CTL‐S and CTL‐X members from different insects could form orthologous groups. In contrast, no T. xiaojinensis IML orthologues were found in other insects. Remarkable lineage‐specific expansion in this subfamily was observed reflecting that these CTLDPs, as important receptors, have evolved diversified members in response to a variety of microbes. Prediction of binding ligands revealed that T. xiaojinensis, a cold‐adapted species, conserved the ability of CRDs to combine with Ca2+ to keep its receptors from freezing. Comparative analysis of induction of CTLDP genes after different immune challenges indicated that IMLs might play critical roles in immune defenses. This study examined T. xiaojinensis CTLDPs and provides a basis for further studies of their characteristics.
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影响因子：
3.8
作者：
Rao, Xiang-Jun;Cao, Xiaolong;He, Yan;Hu, Yingxia;Zhang, Xiufeng;Chen, Yun-Ru;Blissard, Gary;Kanost, Michael R.;Yu, Xiao-Qiang;Jiang, Haobo
通讯作者：
Jiang, Haobo
家蚕 C 型凝集素结构域蛋白 (CTLDP) 的鉴定
影响因子：
2.9
作者：
Rao, Xiang-Jun;Shahzad, Toufeeq;Yu, Xiao-Qiang
通讯作者：
Yu, Xiao-Qiang
影响因子：
2.2
作者：
Koizumi, N;Imai, Y;Sato, R
通讯作者：
Sato, R
影响因子：
4.8
作者：
Graversen, JH;Lorentsen, RH;Etzerodt, M
通讯作者：
Etzerodt, M
用于预防登革热的蚊子 C 型凝集素传播阻断抗体
影响因子：
6.7
作者：
Liu Y;Zhang F;Liu J;Xiao X;Zhang S;Qin C;Xiang Y;Wang P;Cheng G
通讯作者：
Cheng G