Extracellular Vesicles in Lung Cancer: Prospects for Diagnostic and Therapeutic Applications.
Extracellular Vesicles in Lung Cancer: Prospects for Diagnostic and Therapeutic Applications.
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肺癌细胞外囊泡的诊断和治疗应用前景。
DOI：
10.3390/cancers13184604
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发表时间：
2021-09-14
期刊：
影响因子：
5.2
通讯作者：
Hanash S
中科院分区：
文献类型：
--
作者：
Kato T;Vykoukal JV;Fahrmann JF;Hanash S
细胞外囊泡广泛存在于各种生物体液中，含有蛋白质、核酸和脂质等多种生物活性分子。由于脂质双层的包封，它们被认为对相关分子货物提供高稳定性，使其成为液体活检和药物递送系统的理想选择。此外，EV可以影响免疫调节功能，包括抗原呈递和免疫激活和抑制。抑制肿瘤来源的EV的产生可以支持肿瘤免疫，并且免疫细胞来源的EV可以用作抗癌疫苗。本文综述了EV的生物学功能、分离方法及其在肺癌诊断和治疗中的应用。细胞外囊泡是由细胞释放的含有蛋白质、核酸和代谢产物的纳米级脂质结合颗粒。在血液、唾液、痰液和胸腔积液等体液中发现。在肿瘤中，来自癌症和免疫细胞的EV介导细胞间的通讯和交换，并可影响免疫调节功能。在肺癌的背景下，新出现的证据表明EV参与肿瘤发展和进展的各个阶段，包括血管生成、上皮细胞向间质细胞转化、免疫系统抑制、转移和耐药性。此外，肿瘤衍生的EV（TDE）具有作为液体活检源和作为治疗靶向的手段的潜力，并且在这些背景下开发EV的临床应用具有相当大的兴趣。在这篇综述中，我们考虑了EV的生物起源，成分，生物学功能和分离方法，以及它们在肺癌诊断和治疗应用中的临床效用的影响。
Extracellular vesicles (EVs) are extensively distributed in various biological fluids, and contain diverse bioactive molecules including proteins, nucleic acids and lipids. They are considered to provide high stability to the associated molecular cargoes because of encapsulation by the lipid bilayer, making them ideal for liquid biopsy and as a drug delivery system. Moreover, EVs can affect immunomodulatory functions, including antigen presentation and immune activation and suppression. Inhibiting the production of tumor-derived EVs can support tumor immunity, and immune cell-derived EVs can be used as an anticancer vaccine. This review summarizes the biological functions and isolation methods of EVs, and explores their diagnostic and therapeutic applications in lung cancer. Extracellular vesicles (EVs) are nano-sized lipid-bound particles containing proteins, nucleic acids and metabolites released by cells. They have been identified in body fluids including blood, saliva, sputum and pleural effusions. In tumors, EVs derived from cancer and immune cells mediate intercellular communication and exchange, and can affect immunomodulatory functions. In the context of lung cancer, emerging evidence implicates EV involvement during various stages of tumor development and progression, including angiogenesis, epithelial to mesenchymal transformation, immune system suppression, metastasis and drug resistance. Additionally, tumor-derived EVs (TDEs) have potential as a liquid biopsy source and as a means of therapeutic targeting, and there is considerable interest in developing clinical applications for EVs in these contexts. In this review, we consider the biogenesis, components, biological functions and isolation methods of EVs, and the implications for their clinical utility for diagnostic and therapeutic applications in lung cancer.
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DOI：
10.1016/j.jconrel.2015.07.030
发表时间：
2015-12-10
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Batrakova EV;Kim MS
通讯作者：
Kim MS
影响因子：
5.5
作者：
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DOI：
10.1016/j.nano.2017.03.001
发表时间：
2017-07-01
影响因子：
5.4
作者：
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通讯作者：
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影响因子：
7.4
作者：
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通讯作者：
Suzuki, Seigo
影响因子：
7.3
作者：
Gurunathan S;Kang MH;Kim JH
通讯作者：
Kim JH