Interactions between the termini of adeno-associated virus DNA.
Interactions between the termini of adeno-associated virus DNA.
复制标题
腺相关病毒 DNA 末端之间的相互作用。
DOI：
10.1016/0022-2836(89)90526-3
复制
发表时间：
1989
影响因子：
5.6
通讯作者：
Berns,KI
中科院分区：
文献类型：
--
作者：
Bohenzky,RA;Berns,KI
The adeno-associated virus (AAV) genome is a linear, single polynucleotide chain with inverted terminal repeats of 145 bases. In order to test whether the terminal repeats at opposite ends of the genome have to be able to completely base-pair during DNA replication, we have created chimeric genomes in which an 11 base symmetrical sequence has been deleted from the terminal repeat at one end of the genome and replaced by a different 12 base symmetrical sequence. We have used these chimeric constructs either as a duplex insert in pBR322 or as purified duplex virion DNA to transfect adenovirus-infected HeLa cells. When chimeric duplex virion DNA was used, all of the progeny virions obtained after two cell passages contained DNA with wild-type sequences in both terminal repeats. When plasmid clones were used, the structure of virion DNA depended on the original orientation. If the mutant terminal repeat was originally at the right end of the genome (terminus of genetic map), all progeny terminal repeat sequences were again wild-type. However, if the original construct contained the mutant sequence in the left terminal repeat, the majority of progeny molecules were parental in type (i.e. mutant left and wild-type right terminal repeat). We conclude (1) although the terminal repeats at opposite ends of the genome may interact during DNA replication, it is not necessary that they be perfectly complementary. (2) In direct competition, the wild-type sequence displays an advantage over the mutant allele. (3) In a plasmid clone, the terminal repeat on the left end of the genome is at an advantage in a competitive situation. We note that the left terminal repeat is adjacent to a transcriptional promoter.
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具有内部调节域的启动子是 BPV-1 复制起点的一部分。
DOI：
10.1126/science.3037693
发表时间：
1987
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Stenlund,A;Bream,GL;Botchan,MR
通讯作者：
Botchan,MR
腺相关病毒 DNA 复制中构象优先于序列
影响因子：
5.3
作者：
Rance B Lefebvre;S. Riva;K. I. Berns
通讯作者：
K. I. Berns
多瘤病毒增强子重组体的淋巴和其他组织特异性表型：对增强子特异性和活性的正向和负向组合效应
影响因子：
5.3
作者：
B. A. Campbell;L. Villarreal
通讯作者：
L. Villarreal
含有保守序列基序的启动子和增强子元件可被核因子 III（一种刺激腺病毒 DNA 复制的蛋白质）识别。
影响因子：
11.4
作者：
G. Pruijn;W. Driel;R. T. Miltenburg;P. C. Vliet
通讯作者：
P. C. Vliet
氯化镁增强 HeLa 细胞上腺病毒噬斑的形成。
DOI：
10.1099/0022-1317-9-3-251
发表时间：
1970
期刊：
The Journal of general virology
影响因子：
--
作者：
J. Williams
通讯作者：
J. Williams