Spontaneous mutation accumulation in multiple strains of the green alga, Chlamydomonas reinhardtii.
Spontaneous mutation accumulation in multiple strains of the green alga, Chlamydomonas reinhardtii.
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DOI：
10.1111/evo.12448
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发表时间：
2014-09
期刊：
影响因子：
--
通讯作者：
Colegrave N
中科院分区：
文献类型：
--
作者：
Morgan AD;Ness RW;Keightley PD;Colegrave N
对突变参数的估计，如新突变的平均适应度效应和突变产生适应度的新遗传变异的速率，对于理解许多生物过程都很重要。然而，突变参数在种间变化的原因以及它们在物种内变化的程度在很大程度上仍然未知。我们维持了多个单细胞真核生物莱茵衣藻（Chlamydomonas reinhardtii）菌株，通过每~ 10代转移一个细胞，在宽松选择下维持了大约1000代。随着突变世代的积累，品种的平均适应度呈下降趋势，而突变方差呈增加趋势。我们没有发现任何证据表明菌株之间在任何估计的突变参数上存在差异。每次细胞分裂的平均适应度和每次细胞分裂的突变方差输入率的总体变化更接近于在多细胞生物中观察到的值，而不是在其他单细胞微生物中观察到的值。然而，在考虑了物种之间基因组大小的差异之后，多细胞生物和微生物的估计，包括我们对莱茵哈特氏梭菌的新估计，变得更加相似。因此，我们认为基因组大小的变异是突变参数种间变异的重要决定因素。
Estimates of mutational parameters, such as the average fitness effect of a new mutation and the rate at which new genetic variation for fitness is created by mutation, are important for the understanding of many biological processes. However, the causes of interspecific variation in mutational parameters and the extent to which they vary within species remain largely unknown. We maintained multiple strains of the unicellular eukaryote Chlamydomonas reinhardtii, for approximately 1000 generations under relaxed selection by transferring a single cell every ∼10 generations. Mean fitness of the lines tended to decline with generations of mutation accumulation whereas mutational variance increased. We did not find any evidence for differences among strains in any of the mutational parameters estimated. The overall change in mean fitness per cell division and rate of input of mutational variance per cell division were more similar to values observed in multicellular organisms than to those in other single-celled microbes. However, after taking into account differences in genome size among species, estimates from multicellular organisms and microbes, including our new estimates from C. reinhardtii, become substantially more similar. Thus, we suggest that variation in genome size is an important determinant of interspecific variation in mutational parameters.
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DOI：
10.1080/09553005914550241
发表时间：
1959-01-01
期刊：
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY AND RELATED STUDIES IN PHYSICS CHEMISTRY AND MEDICINE
影响因子：
--
作者：
BATEMAN, AJ
通讯作者：
BATEMAN, AJ
影响因子：
56.9
作者：
Davies, EK;Peters, AD;Keightley, PD
通讯作者：
Keightley, PD
影响因子：
3.3
作者：
Joseph, SB;Hall, DW
通讯作者：
Hall, DW
影响因子：
3.3
作者：
Charlesworth, B;Borthwick, H;Pignatelli, P
通讯作者：
Pignatelli, P
DOI：
10.1073/pnas.96.2.574
发表时间：
1999-01-19
影响因子：
11.1
作者：
Fry, JD;Keightley, PD;Nuzhdin, SV
通讯作者：
Nuzhdin, SV