Macroscopic detection of demyelinated lesions in mouse PNS with neutral red dye.
Macroscopic detection of demyelinated lesions in mouse PNS with neutral red dye.
复制标题
DOI：
10.1038/s41598-021-96395-4
复制
发表时间：
2021-08-19
影响因子：
4.6
通讯作者：
Ohno N
中科院分区：
文献类型：
--
作者：
Yamazaki R;Osanai Y;Kouki T;Shinohara Y;Huang JK;Ohno N
溶血磷脂酰胆碱(LPC)诱导的脱髓鞘是一种通用的动物模型，常用于鉴定和研究中枢神经系统(CNS)和周围神经系统(PNS)脱髓鞘和再髓鞘的分子途径。然而，局灶性脱髓鞘病变的识别一直很困难，通常需要组织固定、切片和组织学分析。最近，一种通过腹腔注射中性红(NR)染料来标记和识别中枢神经系统脱髓鞘病变的方法被发展起来。然而，目前尚不清楚NR是否可以用于标记PNS中的脱髓鞘病变。在本研究中，我们建立了LPC诱导的小鼠坐骨神经脱髓鞘，并通过肉眼观察NR标记，证明LPC注射部位的脱髓鞘病变在损伤后7天(DPL)很容易被检测到。此外，在重新髓鞘形成的过程中，NR染色从7dpl逐渐减少到21dpl。7dpl的NR标记的坐骨神经的电子显微镜分析证实了病变中的脱髓鞘和髓鞘碎片。此外，荧光显微镜显示，在三叉神经节损伤处，NR与激活的巨噬细胞和雪旺细胞共同标记。总而言之，NR标记是一种直接的方法，可以对LPC注射后坐骨神经脱髓鞘病变进行宏观检测。
Lysophosphatidylcholine (LPC)-induced demyelination is a versatile animal model that is frequently used to identify and examine molecular pathways of demyelination and remyelination in the central (CNS) and peripheral nervous system (PNS). However, identification of focally demyelinated lesion had been difficult and usually required tissue fixation, sectioning and histological analysis. Recently, a method for labeling and identification of demyelinated lesions in the CNS by intraperitoneal injection of neutral red (NR) dye was developed. However, it remained unknown whether NR can be used to label demyelinated lesions in PNS. In this study, we generated LPC-induced demyelination in sciatic nerve of mice, and demonstrated that the demyelinated lesions at the site of LPC injection were readily detectable at 7 days postlesion (dpl) by macroscopic observation of NR labeling. Moreover, NR staining gradually decreased from 7 to 21 dpl over the course of remyelination. Electron microscopy analysis of NR-labeled sciatic nerves at 7 dpl confirmed demyelination and myelin debris in lesions. Furthermore, fluorescence microscopy showed NR co-labeling with activated macrophages and Schwann cells in the PNS lesions. Together, NR labeling is a straightforward method that allows the macroscopic detection of demyelinated lesions in sciatic nerves after LPC injection.
登录
查看更多内容
DOI：
10.1523/jneurosci.3481-16.2017
发表时间：
2017-04-19
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Benito C;Davis CM;Gomez-Sanchez JA;Turmaine M;Meijer D;Poli V;Mirsky R;Jessen KR
通讯作者：
Jessen KR
影响因子：
7.2
作者：
Salzer, James L.
通讯作者：
Salzer, James L.
DOI：
10.1073/pnas.75.7.3327
发表时间：
1978-01-01
影响因子：
11.1
作者：
OHKUMA, S;POOLE, B
通讯作者：
POOLE, B
DOI：
10.1007/s00018-020-03516-9
发表时间：
2020-10
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Nocera G;Jacob C
通讯作者：
Jacob C
影响因子：
2.5
作者：
KITTELBERGER, R;DAVIS, PF;STEHBENS, WE
通讯作者：
STEHBENS, WE