Limited phylogenetic overlap between fluoroquinolone-resistant Escherichia coli isolated on dairy farms and those causing bacteriuria in humans living in the same geographical region.
Limited phylogenetic overlap between fluoroquinolone-resistant Escherichia coli isolated on dairy farms and those causing bacteriuria in humans living in the same geographical region.
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DOI：
10.1093/jac/dkab310
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发表时间：
2021-11-12
期刊：
影响因子：
--
通讯作者：
Avison MB
中科院分区：
文献类型：
--
作者：
Mounsey O;Schubert H;Findlay J;Morley K;Puddy EF;Gould VC;North P;Bowker KE;Williams OM;Williams PB;Barrett DC;Cogan TA;Turner KM;MacGowan AP;Reyher KK;Avison MB
我们的主要目的是测试奶牛场发现的牛相关的氟喹诺酮耐药（Escherichia coli）大肠杆菌是否与生活在同一个50 × 50 km地理区域内的人类细菌尿症的致病菌密切遗传相关，这表明农场-人类共享。另一个目的是确定存在的风险因素的ERA-RE。奶牛场的大肠杆菌。E.在2017-18年期间，从42个奶牛场和社区尿液样本中分离出大肠杆菌。对42头牛和489株人尿液分离株进行WGS，以进行系统发育比较。使用贝叶斯正则化方法识别风险因素。在489株人源性β-R分离株中，255株也是第三代头孢菌素耐药株，aac（6 '）Ib-cr和blaCTX-M-15之间存在强烈的遗传连锁。我们确定了ST 744和ST 162分离株对可能的农场-人共享，但ST 744和ST 162分离株的农场-人对之间的最小核心基因组SNP距离（分别为71和63个SNP）大于来自不同农场的分离株对之间的最小核心基因组SNP距离（分别为7和3个SNP）。农场氟喹诺酮类药物的总使用量与分离出抗-R E的几率呈正相关。大肠杆菌，而总干奶牛治疗使用显示负相关。这一工作表明，EST-RE。在乳牛场发现的大肠杆菌对当地人群的社区菌尿症影响有限。减少氟喹诺酮类药物的使用可能会降低农场内的RES-RE流行率。当干奶牛治疗针对耐药大肠杆菌的生态学时，这种减少可能更大。农场里的大肠杆菌
Our primary aim was to test whether cattle-associated fluoroquinolone-resistant (FQ-R) Escherichia coli found on dairy farms are closely phylogenetically related to those causing bacteriuria in humans living in the same 50 × 50 km geographical region suggestive of farm–human sharing. Another aim was to identify risk factors for the presence of FQ-R E. coli on dairy farms. FQ-R E. coli were isolated during 2017–18 from 42 dairy farms and from community urine samples. Forty-two cattle and 489 human urinary isolates were subjected to WGS, allowing phylogenetic comparisons. Risk factors were identified using a Bayesian regularization approach. Of 489 FQ-R human isolates, 255 were also third-generation-cephalosporin-resistant, with strong genetic linkage between aac(6’)Ib-cr and blaCTX-M-15. We identified possible farm–human sharing for pairs of ST744 and ST162 isolates, but minimal core genome SNP distances were larger between farm–human pairs of ST744 and ST162 isolates (71 and 63 SNPs, respectively) than between pairs of isolates from different farms (7 and 3 SNPs, respectively). Total farm fluoroquinolone use showed a positive association with the odds of isolating FQ-R E. coli, while total dry cow therapy use showed a negative association. This work suggests that FQ-R E. coli found on dairy farms have a limited impact on community bacteriuria within the local human population. Reducing fluoroquinolone use may reduce the on-farm prevalence of FQ-R E. coli and this reduction may be greater when dry cow therapy is targeted to the ecology of resistant E. coli on the farm.
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影响因子：
5.2
作者：
Pightling AW;Pettengill JB;Luo Y;Baugher JD;Rand H;Strain E
通讯作者：
Strain E
影响因子：
3.9
作者：
Connor TR;Loman NJ;Thompson S;Smith A;Southgate J;Poplawski R;Bull MJ;Richardson E;Ismail M;Thompson SE;Kitchen C;Guest M;Bakke M;Sheppard SK;Pallen MJ
通讯作者：
Pallen MJ
影响因子：
4.9
作者：
Morgan-Linnell, Sonia K.;Zechiedrich, Lynn
通讯作者：
Zechiedrich, Lynn
影响因子：
3.9
作者：
Constantinides, Bede;Chau, Kevin K.;Stoesser, Nicole
通讯作者：
Stoesser, Nicole
影响因子：
4.4
作者：
Schubert, Hannah;Morley, Katy;Reyher, Kristen K.
通讯作者：
Reyher, Kristen K.