Transcriptomic analysis of the differentiating ovary of the protogynous ricefield eel Monopterus albus.
Transcriptomic analysis of the differentiating ovary of the protogynous ricefield eel Monopterus albus.
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原生雌性稻田鳝Monopterus albus卵巢分化的转录组分析
DOI：
10.1186/s12864-017-3953-6
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发表时间：
2017-08-03
期刊：
影响因子：
4.4
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Cai J;Yang W;Chen D;Zhang Y;He Z;Zhang W;Zhang L
稻田鳗鱼是一种雌雄同体的同枝状物种，可以自然地从雌性变为雄性，这为研究脊椎动物的性腺（特别是卵巢）分化提供了一个有趣的模型。本研究通过对稻田鳗鲡幼体性腺的转录组测序，探讨其卵巢分化发育的分子机制。从孵化后6、9、12和20 d的稻田鳗鲡的性腺组织cDNA文库中共获得301,267,988个clean reads，分别包含未分化性腺、分化卵巢、带卵原细胞卵巢和减数分裂卵母细胞卵巢。所有clean reads的De-novo组装共产生265,896个unigenes，平均大小为720 bp， N50为1107 bp。RT-qPCR对13个性腺发育相关功能基因的发育表达分析表明，RNA-seq数据是可靠的。转录组数据表明，稻田鳗鲡幼虫早期性腺中雌性发育相关基因的高表达和雄性发育相关基因的低表达参与了性别分化的级联反应，最终形成雌性表型。黄鳝幼体维甲酸合成与降解相关基因的差异表达模式可能导致黄鳝幼体生殖腺在12 dph时产生维甲酸，并在20 dph时出现减数分裂迹象之前诱导开始减数分裂的分子事件。此外，在稻田鳗鲡幼体性腺转录组数据中只检测到stra6而未检测到stra8，且stra6的表达模式与RA合成相关基因的表达模式相似，提示stra6可能是RA的下游靶点，在稻田鳗鲡幼体的RA代谢和/或减数分裂起始中发挥作用。本研究结果对今后稻田鳗鱼及其他硬骨鱼卵巢分化的研究具有一定的指导意义。
The ricefield eel is a protogynous hermaphroditic Synbranchiform species that changes sex naturally from female to male, which offers an interesting model for studying gonadal (particularly ovarian) differentiation in vertebrates. In the present study, transcriptome sequencing of the gonad of ricefield eel larvae was performed to explore the molecular mechanisms underlying the ovarian differentiation and development. A total of 301,267,988 clean reads were generated from cDNA libraries of gonadal tissues of ricefield eel larvae at 6, 9, 12, and 20 days post hatching (dph), which contained undifferentiated gonads, differentiating ovaries, ovaries with oogonia, and ovaries with meiotic oocytes, respectively. De-novo assembly of all the clean reads generated a total of 265,896 unigenes with a mean size of 720 bp and a N50 of 1107 bp. RT-qPCR analysis of the developmental expression of 13 gonadal development-related functional genes indicated that RNA-seq data are reliable. Transcriptome data suggest that high expression of female development-related genes and low expression of male development-related genes in the early gonads of ricefield eel larvae participate in the cascade of sex differentiation leading to the final female phenotype. The contrasting expression patterns of genes involved in retinoid acid (RA) synthesis and degradation might result in peak production of RA at 12 dph in the gonad of ricefield eel larvae, and induce molecular events responsible for the initiation of meiosis before the meiotic signs could be observed at 20 dph. In addition, only stra6 but not stra8 could be identified in gonadal transcriptome data of ricefield eel larvae, and the expression pattern of stra6 paralleled those of genes involved in RA synthesis, suggesting that stra6 may be a downstream target of RA and play a role in RA metabolism and/or meiotic initiation in the gonad of ricefield eel larvae. Results of the present study will facilitate future study on the ovarian differentiation of ricefield eels and other teleosts as well.
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通讯作者：
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