Long-Term Irrigation Affects the Dynamics and Activity of the Wheat Rhizosphere Microbiome.
Long-Term Irrigation Affects the Dynamics and Activity of the Wheat Rhizosphere Microbiome.
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DOI：
10.3389/fpls.2018.00345
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发表时间：
2018
影响因子：
5.6
通讯作者：
Thomashow LS
中科院分区：
文献类型：
--
作者：
Mavrodi DV;Mavrodi OV;Elbourne LDH;Tetu S;Bonsall RF;Parejko J;Yang M;Paulsen IT;Weller DM;Thomashow LS
内陆太平洋西北地区(IPNW)拥有160万公顷农田，是美国西部的主要小麦产区。整个区域的气候是半干旱的，这使得水的可获得性对IPNW农业来说是一个重大挑战。揭示水分胁迫对小麦生理的影响及其土传病害的动态已引起人们的极大关注。相比之下，土壤水分对旱地小麦根际微生物群落的建立和活动的影响仍然知之甚少。为了解决这一差距，我们进行了一项为期三年的田间研究，涉及华盛顿州林德建立的邻近灌溉和旱地(雨养)地块种植的小麦。我们利用16S rRNA V4区的深度扩增序列分析了小麦根际微生物群对顶灌的响应。我们还研究了本地phz+根际细菌的种群动态和活性，这些细菌产生了抗生素吩嗪-1-羧酸(PCA)，有助于自然抑制小麦土传病原菌。研究结果表明，灌溉对根际细菌phz+的影响是不利的，这从显著降低植物在田间的定植频率、种群大小和PCA水平上可见一斑。在研究过程中，观察到的水浇地和旱地之间的差异是可重复的，并被放大，从而确定土壤水分是影响土著phz+群落动态和活动的关键非生物因素。三个季节的灌溉对根际微生物群落的总体多样性影响不大，但导致特定OTU的相对丰度存在显着差异。特别是，灌溉对多个类杆菌和变形杆菌有不同的影响，包括已知的植物促进生长活性的分类群。对环境变量的分析表明，灌溉和旱地处理之间的分离是由于水势(Ψm)和pH的变化。相反，根际微生物群落组成的时间变化与温度和降水有关。总而言之，我们的长期研究提供了关于半干旱农业生态系统中的水的可用性如何塑造地下小麦微生物群的见解。
The Inland Pacific Northwest (IPNW) encompasses 1. 6 million cropland hectares and is a major wheat-producing area in the western United States. The climate throughout the region is semi-arid, making the availability of water a significant challenge for IPNW agriculture. Much attention has been given to uncovering the effects of water stress on the physiology of wheat and the dynamics of its soilborne diseases. In contrast, the impact of soil moisture on the establishment and activity of microbial communities in the rhizosphere of dryland wheat remains poorly understood. We addressed this gap by conducting a three-year field study involving wheat grown in adjacent irrigated and dryland (rainfed) plots established in Lind, Washington State. We used deep amplicon sequencing of the V4 region of the 16S rRNA to characterize the responses of the wheat rhizosphere microbiome to overhead irrigation. We also characterized the population dynamics and activity of indigenous Phz+ rhizobacteria that produce the antibiotic phenazine-1-carboxylic acid (PCA) and contribute to the natural suppression of soilborne pathogens of wheat. Results of the study revealed that irrigation affected the Phz+ rhizobacteria adversely, which was evident from the significantly reduced plant colonization frequency, population size and levels of PCA in the field. The observed differences between irrigated and dryland plots were reproducible and amplified over the course of the study, thus identifying soil moisture as a critical abiotic factor that influences the dynamics, and activity of indigenous Phz+ communities. The three seasons of irrigation had a slight effect on the overall diversity within the rhizosphere microbiome but led to significant differences in the relative abundances of specific OTUs. In particular, irrigation differentially affected multiple groups of Bacteroidetes and Proteobacteria, including taxa with known plant growth-promoting activity. Analysis of environmental variables revealed that the separation between irrigated and dryland treatments was due to changes in the water potential (Ψm) and pH. In contrast, the temporal changes in the composition of the rhizosphere microbiome correlated with temperature and precipitation. In summary, our long-term study provides insights into how the availability of water in a semi-arid agroecosystem shapes the belowground wheat microbiome.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.4
作者：
Lauber, Christian L.;Hamady, Micah;Fierer, Noah
通讯作者：
Fierer, Noah
影响因子：
3.6
作者：
Glasser NR;Kern SE;Newman DK
通讯作者：
Newman DK
影响因子：
3.2
作者：
Arseneault, Tanya;Goyer, Claudia;Filion, Martin
通讯作者：
Filion, Martin
影响因子：
4.2
作者：
de Boer, Wietse;Wagenaar, Anne-Marieke;van Veen, Johannes A.
通讯作者：
van Veen, Johannes A.