Saccharomyces cerevisiae: population divergence and resistance to oxidative stress in clinical, domesticated and wild isolates.
Saccharomyces cerevisiae: population divergence and resistance to oxidative stress in clinical, domesticated and wild isolates.
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DOI：
10.1371/journal.pone.0005317
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Dietrich FS
中科院分区：
文献类型：
--
作者：
Diezmann S;Dietrich FS
酿酒酵母在酿酒厂和面包店中与人类生活联系了数千年。最近它被认为是一种新出现的机会致病菌。为了研究S.酿酒酵母，临床分离物的起源和毒性相关性状的重要性，群体遗传学和表型测定已应用于从诊所，啤酒厂，葡萄园，水果，土壤，商业补充剂和昆虫内脏分离的103种菌株的生态多样性组。从五个核DNA位点的DNA序列数据进行了分析，人口结构和单倍型分布。此外，测试所有菌株的氧化应激存活，这是一种与微生物致病性相关的性状。DNA序列分析确定了重组S.酿酒酵母菌株与生态、地理和毒力有关。共享等位基因表明，临床分离物含有来自水果分离物的遗传贡献。临床和果实分离株表现出高水平的重组，不像遗传同质的土壤分离株，其中没有重组被检测到。然而，临床和土壤分离株比任何其他人群更耐氧化应激，这表明在氧化应激和酵母致病性之间的生存相关性。群体遗传学分析表明，S.酿酒酵母划分了三个不同的群体，主要包括（i）人类相关的酿酒厂和葡萄园菌株，（ii）临床和果实分离株（iii）和来自美国东部的野生土壤分离株。酿酒酵母和人类的共同作用促进了酵母的进化和遗传、生态和地理上不同群体的发展。
Saccharomyces cerevisiae has been associated with human life for millennia in the brewery and bakery. Recently it has been recognized as an emerging opportunistic pathogen. To study the evolutionary history of S. cerevisiae, the origin of clinical isolates and the importance of a virulence-associated trait, population genetics and phenotypic assays have been applied to an ecologically diverse set of 103 strains isolated from clinics, breweries, vineyards, fruits, soil, commercial supplements and insect guts. DNA sequence data from five nuclear DNA loci were analyzed for population structure and haplotype distribution. Additionally, all strains were tested for survival of oxidative stress, a trait associated with microbial pathogenicity. DNA sequence analyses identified three genetic subgroups within the recombining S. cerevisiae strains that are associated with ecology, geography and virulence. Shared alleles suggest that the clinical isolates contain genetic contribution from the fruit isolates. Clinical and fruit isolates exhibit high levels of recombination, unlike the genetically homogenous soil isolates in which no recombination was detected. However, clinical and soil isolates were more resistant to oxidative stress than any other population, suggesting a correlation between survival in oxidative stress and yeast pathogenicity. Population genetic analyses of S. cerevisiae delineated three distinct groups, comprising primarily the (i) human-associated brewery and vineyard strains, (ii) clinical and fruit isolates (iii) and wild soil isolates from eastern U.S. The interactions between S. cerevisiae and humans potentiate yeast evolution and the development of genetically, ecologically and geographically divergent groups.
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影响因子：
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作者：
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通讯作者：
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