The response and recovery of the Arabidopsis thaliana transcriptome to phosphate starvation.
The response and recovery of the Arabidopsis thaliana transcriptome to phosphate starvation.
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DOI：
10.1186/1471-2229-12-62
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发表时间：
2012-05-03
影响因子：
5.3
通讯作者：
Chua NH
中科院分区：
文献类型：
--
作者：
Woo J;MacPherson CR;Liu J;Wang H;Kiba T;Hannah MA;Wang XJ;Bajic VB;Chua NH
现代农业中磷肥的过量施用污染了水道，破坏了自然生态系统。然而，这是农民的普遍做法，特别是在发展中国家，因为人们认为大量的肥料可以提高作物产量。植物磷代谢及其潜在遗传途径的研究是发现有效肥料使用方法的关键。这里介绍的工作描述了一个全基因组资源的分子动力学基础的响应和恢复拟南芥根和芽磷酸盐饥饿。微阵列和平铺阵列（可从GEO：GSE 34004）的全基因组分析显示，根和芽转录组之间的重叠最小，这表明两个独立的磷酸盐饥饿调节子。新的基因表达模式被检测到超过1000名候选人，并被分类为初始，持续或潜在的反应。与AtGenExpress的比较分析鉴定了跨多种刺激共调节的基因的群组。激素阿坝在调节许多磷酸盐响应候选人中显示出主导作用。共调节的分析，使特定与密切相关的基因家族的成员与磷饥饿的确定。因此，除其他外，我们表明，PHR 1调节密切相关的磷酸盐响应家族（PHT 1;1，PHT 1;7-9，SPX 1 -3和PHO 1;H1）的成员显示更大的特异性磷酸盐饥饿比他们更一般的同行。我们的研究结果揭示了更大的，阶段性的反应比以前描述的磷酸盐饥饿。据我们所知，这项工作描述了迄今为止最完整的植物营养胁迫全基因组数据。
Over application of phosphate fertilizers in modern agriculture contaminates waterways and disrupts natural ecosystems. Nevertheless, this is a common practice among farmers, especially in developing countries as abundant fertilizers are believed to boost crop yields. The study of plant phosphate metabolism and its underlying genetic pathways is key to discovering methods of efficient fertilizer usage. The work presented here describes a genome-wide resource on the molecular dynamics underpinning the response and recovery in roots and shoots of Arabidopsis thaliana to phosphate-starvation. Genome-wide profiling by micro- and tiling-arrays (accessible from GEO: GSE34004) revealed minimal overlap between root and shoot transcriptomes suggesting two independent phosphate-starvation regulons. Novel gene expression patterns were detected for over 1000 candidates and were classified as either initial, persistent, or latent responders. Comparative analysis to AtGenExpress identified cohorts of genes co-regulated across multiple stimuli. The hormone ABA displayed a dominant role in regulating many phosphate-responsive candidates. Analysis of co-regulation enabled the determination of specific versus generic members of closely related gene families with respect to phosphate-starvation. Thus, among others, we showed that PHR1-regulated members of closely related phosphate-responsive families (PHT1;1, PHT1;7–9, SPX1-3, and PHO1;H1) display greater specificity to phosphate-starvation than their more generic counterparts. Our results uncover much larger, staged responses to phosphate-starvation than previously described. To our knowledge, this work describes the most complete genome-wide data on plant nutrient stress to-date.
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影响因子：
10.7
作者：
Hanada K;Sawada Y;Kuromori T;Klausnitzer R;Saito K;Toyoda T;Shinozaki K;Li WH;Hirai MY
通讯作者：
Hirai MY
影响因子：
46.9
作者：
Ji, Hongkai;Jiang, Hui;Ma, Wenxiu;Johnson, David S.;Myers, Richard M.;Wong, Wing H.
通讯作者：
Wong, Wing H.
DOI：
10.1073/pnas.95.4.1950
发表时间：
1998-02-17
影响因子：
11.1
作者：
Essigmann, B;Güler, S;Benning, C
通讯作者：
Benning, C
影响因子：
8.8
作者：
Ma, Wenqi;Ma, Lin;Zhang, Fusuo
通讯作者：
Zhang, Fusuo
DOI：
10.1073/pnas.0505266102
发表时间：
2005-08-16
影响因子：
11.1
作者：
Misson, J;Raghothama, KG;Thibaud, MC
通讯作者：
Thibaud, MC