Enzyme-linked immunosorbent-assay for Deoxynivalenol (DON).
Enzyme-linked immunosorbent-assay for Deoxynivalenol (DON).
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作者：
Ji F;Li H;Xu J;Shi J
脱氧雪腐镰刀菌烯醇（DON）是一种具有生物活性的真菌毒素，是小麦和大麦的主要污染物，尤其是在小麦赤霉病的病原菌禾谷镰刀菌（Fusarium graminearum）的侵染下。由于DON摄入的高风险和频繁暴露的可能性，重要的是开发一种快速，高灵敏度的方法，易于识别和定量的DON在粮食样品中。本研究建立了间接竞争酶联免疫吸附试验（ELISA）检测小麦中DON的方法。将3-O-半琥珀酰-DON（3 HS-DON）分别与牛血清白蛋白（BSA）和卵清蛋白（OVA）偶联，制备了DON特异性抗血清。间接竞争ELISA试验表明，小鼠血清和包被抗原的最适浓度分别为1/1600和1/1500。该抗血清与3-乙酰-DON和T-2毒素的交叉反应率分别为DON的55.2%和6.3%。结果表明，使用含有低于15%甲醇的提取缓冲液可令人满意地进行含量测定。谷物中DON的回收率为82-93%。谷物中DON的线性检测范围为0.01 ~ 100 μg/mL。
Deoxynivalenol (DON), one of the trichothecene mycotoxins, is a worldwide contaminant of wheat and barley, especially when infected by Fusarium graminearum, the causative agent of an epidemic wheat disease called Fusarium Head Blight. Because of the high risk of DON ingestion and the possibility of frequent exposure, it is important to develop a rapid and highly sensitive method for easy identification and quantification of DON in grain samples. In this study, we have developed an indirect competitive enzyme-linked immunosorbent assay (ELISA) to detect DON in wheat. We conjugated 3-O-Hemisuccinyl-DON (3HS-DON) to Bovine serum albumin (BSA) and Ovalbumin (OVA), and obtained DON-specific mice antisera. The indirect competitive ELISA revealed that the optimal concentration of mice serum and the coated antigen was 1/1600 and 1/1500, respectively. The antiserum cross-reacted with the trichothecenes 3-acetyl-DON and T-2 toxin, reaching about 55.2% and 6.3%, respectively, as compared with DON. Results showed that the assay could be performed satisfactorily using an extraction buffer containing less than 15% methanol. Recovery from DON was 82–93% in grains. The linear detection range of DON in grains was between 0.01 and 100 μg/mL.
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影响因子：
3
作者：
Alexander, Nancy J.;McCormick, Susan P.;Proctor, Robert H.
通讯作者：
Proctor, Robert H.
影响因子：
2.7
作者：
UENO, Y;HOSOYA, M;TATSUNO, T
通讯作者：
TATSUNO, T
谷物中的毛thothecenes。
影响因子：
5.6
作者：
Foroud NA;Eudes F
通讯作者：
Eudes F
DOI：
10.1016/0022-4731(75)90141-7
发表时间：
1975-01-01
影响因子：
4.1
作者：
ABRAHAM, GE
通讯作者：
ABRAHAM, GE
影响因子：
3.5
作者：
Pestka, James J.;Islam, Zahidul;Amuzie, Chidozie J.
通讯作者：
Amuzie, Chidozie J.