Development of a high-throughput starch digestibility assay reveals wide variation among the A. E. Watkins wheat landrace collection
Development of a high-throughput starch digestibility assay reveals wide variation among the A. E. Watkins wheat landrace collection
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高通量淀粉消化率测定的开发揭示了 A. E. Watkins 小麦地方品种之间的巨大差异
DOI:
10.1101/2022.11.04.515016
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发表时间:
2022
期刊:
影响因子:
--
通讯作者:
Zafeiriou P
中科院分区:
文献类型:
--
作者:
Zafeiriou P
Breeding for less digestible starch in wheat can improve the health impact of bread and other wheat foods. Based on an establishedin vitrostarch digestibility assay by we developed a high-throughput assay to measure starch digestibility in hydrothermally processed samples for use in forward genetic approaches. Digestibility of purified starch from maize and wheat was measured using both methods and produced comparable results. Using the high-throughput assay, we estimated starch digestibility of 118 wheat landraces from the core Watkins collection and found wide variation across lines and elite UK varieties, (20% to 40% and 31% to 44% starch digested after 90 minutes respectively). Sieved flour fractions and purified starch for selected lines showed altered starch digestibility profiles compared with wholemeal flour, suggesting that matrix properties of flour rather than intrinsic properties of starch granules conferred the low starch digestibility observed.
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