Development of a high-throughput starch digestibility assay reveals wide variation among the A. E. Watkins wheat landrace collection

Development of a high-throughput starch digestibility assay reveals wide variation among the A. E. Watkins wheat landrace collection
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高通量淀粉消化率测定的开发揭示了 A. E. Watkins 小麦地方品种之间的巨大差异

DOI:
10.1101/2022.11.04.515016
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发表时间:
2022
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通讯作者:
Zafeiriou P
Zafeiriou P
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作者:
Zafeiriou P

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培育小麦中较难消化的淀粉可以改善面包和其他小麦食品对健康的影响。基于建立的试管淀粉消化率测定方法,我们建立了一种高通量测定水热处理样品中淀粉消化率的方法,用于正向遗传方法。用这两种方法测定了玉米和小麦精制淀粉的消化率,结果具有可比性。使用高通量分析方法,我们估计了来自Watkins核心种质的118个小麦地方品种的淀粉消化率,发现不同品系和英国优良品种之间的淀粉消化率存在很大差异(90分钟后淀粉消化率分别为20%~40%和31%~44%)。与全麦粉相比,筛选出的面粉组分和精制淀粉的淀粉消化率曲线发生了变化,表明淀粉消化率低的原因是面粉的基质特性,而不是淀粉颗粒的固有特性。
Breeding for less digestible starch in wheat can improve the health impact of bread and other wheat foods. Based on an establishedin vitrostarch digestibility assay by we developed a high-throughput assay to measure starch digestibility in hydrothermally processed samples for use in forward genetic approaches. Digestibility of purified starch from maize and wheat was measured using both methods and produced comparable results. Using the high-throughput assay, we estimated starch digestibility of 118 wheat landraces from the core Watkins collection and found wide variation across lines and elite UK varieties, (20% to 40% and 31% to 44% starch digested after 90 minutes respectively). Sieved flour fractions and purified starch for selected lines showed altered starch digestibility profiles compared with wholemeal flour, suggesting that matrix properties of flour rather than intrinsic properties of starch granules conferred the low starch digestibility observed.
五种小麦淀粉分支酶 II 基因的组合突变可提高抗性淀粉,但会影响谷物产量和面包制作质量
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