Efficacy of various disinfectants against SARS coronavirus.
Efficacy of various disinfectants against SARS coronavirus.
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DOI：
10.1016/j.jhin.2004.12.023
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发表时间：
2005-10
期刊：
影响因子：
--
通讯作者：
Doerr HW
中科院分区：
文献类型：
--
作者：
Rabenau HF;Kampf G;Cinatl J;Doerr HW
最近在亚洲和北美流行的严重急性呼吸系统综合症（SARS）导致广泛使用各种消毒剂，以控制高度传染性病毒的公众传播。然而，只有有限的数据可以证明它们对SARS冠状病毒（SARS- cov）的有效性。因此，我们根据prEN 14476检测了8种消毒剂对SARS-CoV的活性。在30秒内测试了四种手部摩擦剂（Sterillium，基于45%异丙醇，30%正丙醇和0.2%乙酸甲啶铵；Sterillium Rub，基于80%乙醇；Sterillium Gel，基于85%乙醇；Sterillium Virugard，基于95%乙醇）。研究了三种表面消毒剂，浓度为0.5%，作用时间为30 min和60 min（以苯扎氯铵和十二胺为基础的Mikrobac forte；以苯扎氯铵、戊二醛和二烷基氯二铵为基础的Kohrsolin FF；以单邻苯二甲酸镁为基础的Dismozon pur），以及一种仪器消毒剂，浓度为4%，作用时间为15 min， 3%，作用时间为30 min， 2%，作用时间为60 min [Korsolex碱性，以戊二醛和（乙二氧基）二甲醇为基础]。采用三种有机负荷：0.3%白蛋白、10%胎牛血清和0.3%白蛋白加0.3%绵羊红细胞。病毒滴度采用96孔微滴板定量检测（终点滴定法）。所有检测的制剂，无论何种有机负荷，SARS-CoV均被灭活至低于检测限（还原因子大多≥4）。总之，sars冠状病毒可以很容易地用许多常用的消毒剂灭活。
The recent severe acute respiratory syndrome (SARS) epidemic in Asia and Northern America led to broad use of various types of disinfectant in order to control the public spread of the highly contagious virus. However, only limited data were available to demonstrate their efficacy against SARS coronavirus (SARS-CoV). We therefore investigated eight disinfectants for their activity against SARS-CoV according to prEN 14476. Four hand rubs were tested at 30 s (Sterillium, based on 45% iso-propanol, 30% n-propanol and 0.2% mecetronium etilsulphate; Sterillium Rub, based on 80% ethanol; Sterillium Gel, based on 85% ethanol; Sterillium Virugard, based on 95% ethanol). Three surface disinfectants were investigated at 0.5% for 30 min and 60 min (Mikrobac forte, based on benzalkonium chloride and laurylamine; Kohrsolin FF, based on benzalkonium chloride, glutaraldehyde and didecyldimonium chloride; Dismozon pur, based on magnesium monoperphthalate), and one instrument disinfectant was investigated at 4% for 15 min, 3% for 30 min and 2% for 60 min [Korsolex basic, based on glutaraldehyde and (ethylenedioxy)dimethanol]. Three types of organic load were used: 0.3% albumin, 10% fetal calf serum, and 0.3% albumin with 0.3% sheep erythrocytes. Virus titres were determined by a quantitative test (endpoint titration) in 96-well microtitre plates. With all tested preparations, SARS-CoV was inactivated to below the limit of detection (reduction factor mostly ≥4), regardless of the type of organic load. In summary, SARS-CoV can be inactivated quite easily with many commonly used disinfectants.
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DOI：
10.1016/s0140-6736(03)15259-2
发表时间：
2004-01-10
期刊：
Lancet (London, England)
影响因子：
--
作者：
Guan Y;Peiris JS;Zheng B;Poon LL;Chan KH;Zeng FY;Chan CW;Chan MN;Chen JD;Chow KY;Hon CC;Hui KH;Li J;Li VY;Wang Y;Leung SW;Yuen KY;Leung FC
通讯作者：
Leung FC
影响因子：
6.9
作者：
Darbord, JC
通讯作者：
Darbord, JC
影响因子：
6.9
作者：
Payan, C;Pivert, A;Lemarie, C
通讯作者：
Lemarie, C
影响因子：
5.4
作者：
Rabenau HF;Cinatl J;Morgenstern B;Bauer G;Preiser W;Doerr HW
通讯作者：
Doerr HW
影响因子：
8.8
作者：
Manocha, S;Walley, KR;Russell, JA
通讯作者：
Russell, JA