Stability and inactivation of SARS coronavirus.
Stability and inactivation of SARS coronavirus.
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DOI：
10.1007/s00430-004-0219-0
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发表时间：
2005-01
影响因子：
5.4
通讯作者：
Doerr HW
中科院分区：
文献类型：
--
作者：
Rabenau HF;Cinatl J;Morgenstern B;Bauer G;Preiser W;Doerr HW
SARS冠状病毒（SARS-CoV）是一种新出现的高致病性病原体，在2002年11月至2003年9月期间造成8，000多人感染，近800人死亡。虽然大多数病例是通过呼吸道飞沫直接人际传播，但并不排除其他模式。粪便脱落是常见和长期的，并导致在香港爆发。我们研究了SARS-CoV在不同条件下的稳定性，无论是在悬浮液中还是在表面干燥，与其他人类致病性病毒，包括人类冠状病毒HCoV-229 E进行比较。在悬浮液中，HCoV-229 E逐渐完全失去其感染性，而SARS-CoV保留其感染性长达9天;在干燥状态下，存活时间为24小时与6天。在不存在蛋白质的情况下，56°C下的热灭活非常有效，将病毒滴度降低至低于可检测性;然而，添加20%蛋白质产生保护作用，导致残留感染性。如果要灭活含蛋白质的溶液，必须在60°C下热处理至少30分钟。不同的固定程序，例如用于制备免疫荧光载玻片，以及医院和实验室环境中常用的病毒灭活化学方法通常被认为是有效的。我们的研究证实，有可能照顾SARS患者，并安全地进行SARS-CoV的实验室科学研究。然而，该病原体的韧性比HCoV-229 E高得多，如果SARS再次出现，需要加大力度解决环境卫生问题。
The SARS-coronavirus (SARS-CoV) is a newly emerged, highly pathogenic agent that caused over 8,000 human infections with nearly 800 deaths between November 2002 and September 2003. While direct person-to-person transmission via respiratory droplets accounted for most cases, other modes have not been ruled out. Faecal shedding is common and prolonged and has caused an outbreak in Hong Kong. We studied the stability of SARS-CoV under different conditions, both in suspension and dried on surfaces, in comparison with other human-pathogenic viruses, including human coronavirus HCoV-229E. In suspension, HCoV-229E gradually lost its infectivity completely while SARS-CoV retained its infectivity for up to 9 days; in the dried state, survival times were 24 h versus 6 days. Thermal inactivation at 56°C was highly effective in the absence of protein, reducing the virus titre to below detectability; however, the addition of 20% protein exerted a protective effect resulting in residual infectivity. If protein-containing solutions are to be inactivated, heat treatment at 60°C for at least 30 min must be used. Different fixation procedures, e.g. for the preparation of immunofluorescence slides, as well as chemical means of virus inactivation commonly used in hospital and laboratory settings were generally found to be effective. Our investigations confirm that it is possible to care for SARS patients and to conduct laboratory scientific studies on SARS-CoV safely. Nevertheless, the agent’s tenacity is considerably higher than that of HCoV-229E, and should SARS re-emerge, increased efforts need to be devoted to questions of environmental hygiene.
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影响因子：
13.6
作者：
Drosten C;Preiser W;Günther S;Schmitz H;Doerr HW
通讯作者：
Doerr HW
DOI：
10.1016/s0140-6736(03)13168-6
发表时间：
2003-05-03
期刊：
Lancet (London, England)
影响因子：
--
作者：
Seto WH;Tsang D;Yung RW;Ching TY;Ng TK;Ho M;Ho LM;Peiris JS;Advisors of Expert SARS group of Hospital Authority
通讯作者：
Advisors of Expert SARS group of Hospital Authority
DOI：
10.1053/jhin.2000.0795
发表时间：
2000-09
期刊：
The Journal of hospital infection
影响因子：
--
作者：
Sizun J;Yu MW;Talbot PJ
通讯作者：
Talbot PJ
影响因子：
5.6
作者：
Snijder EJ;Bredenbeek PJ;Dobbe JC;Thiel V;Ziebuhr J;Poon LL;Guan Y;Rozanov M;Spaan WJ;Gorbalenya AE
通讯作者：
Gorbalenya AE
DOI：
10.1016/s0140-6736(03)13412-5
发表时间：
2003-05-24
期刊：
Lancet (London, England)
影响因子：
--
作者：
Peiris JS;Chu CM;Cheng VC;Chan KS;Hung IF;Poon LL;Law KI;Tang BS;Hon TY;Chan CS;Chan KH;Ng JS;Zheng BJ;Ng WL;Lai RW;Guan Y;Yuen KY;HKU/UCH SARS Study Group
通讯作者：
HKU/UCH SARS Study Group