Comprehensive QTL analyses of nitrogen use efficiency in indica rice.
Comprehensive QTL analyses of nitrogen use efficiency in indica rice.
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籼稻氮素利用效率QTL综合分析
DOI：
10.3389/fpls.2022.992225
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
水稻氮素利用效率（NUE）是一个涉及多种生物学过程和农艺性状的复杂数量性状，其遗传基础和调控网络尚不清楚。我们构建了一个高分辨率的基因芯片为基础的遗传图谱，261重组自交系来自两个籼稻亲本。利用2,345个bin标记，对两个不同氮水平下7个主要农艺性状的QTL进行了综合分析。共检测到有效穗数（EPN）非冗余QTL 11个，每穗粒数7个，千粒重13个，结实率2个，株高15个，穗长12个，单株产量6个。QTL区域平均为512 kb，其中超过一半的QTL区间小于100 kb。利用这一优势，我们确定了两个可能的候选基因的主要EPN相关的QTL。在两个氮水平下检测到的一个QTL可能编码DELLA蛋白SLR 1，该蛋白已知调节NUE，尽管该蛋白的天然变异尚未报道。另一个QTL仅在高氮水平下检测到，编码转录因子OsbZIP 59。我们还预测了另外三个与NUE相关的QTL的候选基因。本研究结果为进一步完善籼型水稻NUE相关QTL的克隆提供了参考，并有助于加深对籼型水稻NUE调控的认识。
Nitrogen-use efficiency (NUE) in rice is a complex quantitative trait involved in multiple biological processes and agronomic traits; however, the genetic basis and regulatory network of NUE remain largely unknown. We constructed a high-resolution microarray-based genetic map for 261 recombinant inbred lines derived from two indica parents. Using 2,345 bin markers, comprehensive analyses of quantitative trait loci (QTLs) of seven key agronomic traits under two different N levels were performed. A total of 11 non-redundant QTLs for effective panicle number (EPN), 7 for grain number per panicle, 13 for thousand-grain weight, 2 for seed-setting percentage, 15 for plant height, 12 for panicle length, and 6 for grain yield per plant were identified. The QTL regions were as small as 512 kb on average, and more than half spanned an interval smaller than 100 kb. Using this advantage, we identified possible candidate genes of two major EPN-related QTLs. One QTL detected under both N levels possibly encodes a DELLA protein SLR1, which is known to regulate NUE, although the natural variations of this protein have not been reported. The other QTL detected only under a high N level could encode the transcription factor OsbZIP59. We also predicted the possible candidate genes for another three of the NUE-related QTLs. Our results provide a reference for improving NUE-related QTL cloning and promote our understanding of NUE regulation in indica rice.
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