Strengthening the potential of biomineralized microspheres in enhancing osteogenesis via incorporating alendronate
Strengthening the potential of biomineralized microspheres in enhancing osteogenesis via incorporating alendronate
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通过掺入阿仑膦酸钠增强生物矿化微球增强成骨的潜力
DOI：
10.1016/j.cej.2019.02.202
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发表时间：
2019-07
影响因子：
15.1
通讯作者：
Xiaoping Yang
中科院分区：
文献类型：
--
作者：
Pengfei Wei;Zuoying Yuan;Wei Jing;Yiqian Huang;Qing Cai;Binbin Guan;Zihao Liu;Xu Zhang;Jianping Mao;Dafu Chen;Xiaoping Yang
Alendronate, a nitrogen-containing bisphosphate, is commonly used for the treatment of osteoporosis, showing the potential in enhancing osteogenesis. To improve the osteo-promoting effect of injectable biomineralized microspheres for bone tissue engineering applications, in this study, alendronate was incorporated onto the microspheres during their biomineralization procedure in simulated body fluid (SBF). For the purpose, the microspheres were made of biodegradable poly(l-lactide)-poly(ethylene glycol) block copolymer, which had rough surface with gelatin coating via the (W1/O)/W2emulsion preparation method. Different amounts (25 μM, 50 μM or 100 μM) of alendronate were dissolved in the 5-times SBF and incorporated as calcium minerals depositing onto the microspheres, and thus displaying different release behaviors of alendronate. The cell culture results using bone marrow mesenchymal stromal cells (BMSCs) demonstrated that the microspheres prepared from the 50 μM of alendronate had the strongest promotion on osteogenic differentiation, while displayed insignificant adverse effect on cell proliferation and viability. By adding Noggin (an inhibitor for bone morphogenetic protein (BMP)) into the culture systems, it was proposed that BMP signaling pathway was one of the possible pathways for alendronate playing its activating effect. In vivo evaluation was conducted by injecting the alendronate-loaded microspheres subcutaneously into rabbit back, in which, histological and immunohistological analysis were done to assess ectopic neovascularization and osteogenesis. Promisingly, the potential of biomineralized microspheres in promoting osteogenesis was significantly strengthened with the incorporation of alendronate. Therefore, a proper application of alendronate in bone repairing materials can be an effective strategy targeting bone regeneration.
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影响因子：
9.7
作者：
Luo, Xiaoman;Barbieri, Davide;Bruijn, Joost D.
通讯作者：
Bruijn, Joost D.
影响因子：
3.4
作者：
Shao-qiong Zhou;X. Fang;Huaping Xin;Si-ming Guan
通讯作者：
Shao-qiong Zhou;X. Fang;Huaping Xin;Si-ming Guan
影响因子：
2.6
作者：
Kang AR;Oh YR;Kim HY;Park MJ;Joo BS;Choi WJ;Lee JY;Jung MH;Ji YI;Choi JS
通讯作者：
Choi JS
DOI：
--
发表时间：
2002
期刊：
--
影响因子：
--
作者：
J. Kuljanin
通讯作者：
J. Kuljanin
影响因子：
9.5
作者：
Tarafder, Solaiman;Bose, Susmita
通讯作者：
Bose, Susmita