Optoelectronic control of surface charge and translocation dynamics in solid-state nanopores.
Optoelectronic control of surface charge and translocation dynamics in solid-state nanopores.
复制标题
DOI：
10.1038/nnano.2013.221
复制
发表时间：
2013-12
影响因子：
38.3
通讯作者：
中科院分区：
文献类型：
--
作者：
纳米孔可用于检测和分析生物分子。然而，控制和调节分子通过孔隙的易位速度很困难，限制了这些传感器的更广泛应用。在这里，我们证明低功率可见光可用于控制固态纳米孔中的表面电荷，并可影响 DNA 和蛋白质的易位动力学。我们发现，精确聚焦在纳米孔上的激光可以诱导高达 1 C/m2 的可逆负表面电荷密度，并且通过调整光子密度，可以在亚毫秒时间尺度上调节该效应。通过调节表面电荷，我们可以控制通过纳米孔的电渗流量，从而影响生物分子移位的速度。特别是，几毫瓦的绿光可以使双链DNA的易位速度降低一个数量级以上，使泛素等小球状蛋白的易位速度降低两个数量级以上。激光还可用于疏通堵塞的毛孔。最后，我们讨论了一种解释所观察到的光电现象的机制。
Nanopores can be used to detect and analyse biomolecules. However, controlling and tuning the translocation speed of molecules through a pore is difficult, limiting the wider application of these sensors. Here we show that low-power visible light can be used to control surface charge in solid-state nanopores and can influence the translocation dynamics of DNA and proteins. We find that laser light precisely focused at a nanopore can induce reversible negative surface charge densities as high as 1 C/m2, and that the effect is tuneable on sub-millisecond timescales by adjusting the photon density. By modulating surface charge, we can control the amount of electro-osmotic flow through the nanopore, which affects the speed of translocating biomolecules. In particular, a few mW of green light can reduce the translocation speed of double-stranded DNA by more than an order of magnitude and the translocation speed of small globular proteins such as ubiquitin by more than two orders of magnitude. The laser light can also be used to unclog blocked pores. Finally, we discuss a mechanism to account for the observed optoelectronic phenomenon.
登录
查看更多内容
影响因子：
10.8
作者：
Nam, Sung-Wook;Rooks, Michael J.;Rossnagel, Stephen M.
通讯作者：
Rossnagel, Stephen M.
影响因子：
2.4
作者：
Jiang, Zhijun;Stein, Derek
通讯作者：
Stein, Derek
影响因子：
10.8
作者：
Fologea, D;Uplinger, J;Li, JL
通讯作者：
Li, JL
DOI：
10.1073/pnas.0500796102
发表时间：
2005-07-26
影响因子：
11.1
作者：
Ho, C;Qiao, R;Timp, G
通讯作者：
Timp, G
影响因子：
10.8
作者：
Singer A;Rapireddy S;Ly DH;Meller A
通讯作者：
Meller A