Oviduct epithelium induces interferon-tau in bovine Day-4 embryos, which generates an anti-inflammatory response in immune cells.
Oviduct epithelium induces interferon-tau in bovine Day-4 embryos, which generates an anti-inflammatory response in immune cells.
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DOI：
10.1038/s41598-018-26224-8
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发表时间：
2018-05-18
影响因子：
4.6
通讯作者：
Miyamoto A
中科院分区：
文献类型：
--
作者：
Talukder AK;Rashid MB;Yousef MS;Kusama K;Shimizu T;Shimada M;Suarez SS;Imakawa K;Miyamoto A
最近的研究表明，牛胚胎和母亲之间的交流是从输卵管开始的。在这里，我们的目的是利用体外模型研究胚胎对牛输卵管免疫反应的影响。首先，当胚胎达到16个细胞阶段时，将受精卵与牛输卵管上皮细胞（BOECs）一起或不一起培养4天。当时，我们在与boec共培养的胚胎中检测到干扰素-tau (IFNT)，但在单独培养的胚胎中未检测到。接下来，外周血单个核细胞（PBMCs）在胚胎单独培养或胚胎与boec共培养的培养基中孵育。单独培养胚的培养基不调节PBMCs基因的表达；而胚胎- boec共培养培养基增加了干扰素刺激基因（ISGs: ISG15, OAS1, MX2）， STAT1， PTGES和TGFB1，但抑制了pbmc中IL17的表达。ifnt处理的BOEC培养基和单独添加ifnt的不含BOEC的新鲜培养基对PBMCs基因表达的调节作用与胚胎-BOEC共培养培养基相似。此外，IFNT特异性抗体可中和胚胎- boec共培养基对PBMCs基因表达的影响。我们的研究结果表明，boec刺激胚胎产生IFNT， IFNT随后作用于免疫细胞，促进输卵管内的抗炎反应。
Recent studies indicate that communication between the bovine embryo and the mother begins in the oviduct. Here, we aimed to investigate the effect of embryos on bovine oviducts for their immune responses using an in vitro model. First, zygotes were cultured with or without bovine oviduct epithelial cells (BOECs) for 4 days, when embryos had reached the 16-cell stage. At that time, we detected interferon-tau (IFNT) in embryos co-cultured with BOECs, but not in embryos cultured alone. Next, peripheral blood mononuclear cells (PBMCs) were incubated either in media from embryo alone cultures or from co-cultures of embryos with BOECs. The medium from embryo alone cultures did not modulate PBMCs gene expression; whereas the embryo-BOEC co-culture medium increased interferon-stimulated genes (ISGs: ISG15, OAS1, MX2), STAT1, PTGES and TGFB1 but suppressed IL17 expression in PBMCs. Both IFNT-treated BOEC culture medium and IFNT-supplemented fresh medium alone without BOEC, modulated PBMCs gene expressions similar to those by the embryo-BOEC co-culture medium. Further, specific antibody to IFNT neutralized the effect of embryo-BOEC co-culture medium on PBMCs gene expression. Our results indicate that BOECs stimulate embryos to produce IFNT, which then acts on immune cells to promote an anti-inflammatory response in the oviduct.
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DOI：
10.1262/jrd.2013-036
发表时间：
2013-10
期刊：
The Journal of reproduction and development
影响因子：
--
作者：
Kowsar R;Hambruch N;Liu J;Shimizu T;Pfarrer C;Miyamoto A
通讯作者：
Miyamoto A
影响因子：
64.8
作者：
Bettelli, E;Carrier, YJ;Kuchroo, VK
通讯作者：
Kuchroo, VK
影响因子：
4.6
作者：
Ideta, Atsushi;Aoyagi, Yoshito;Tsuchiya, Kanami;Kamijima, Tatsuro;Nishimiya, Yoshiyuki;Tsuda, Sakae
通讯作者：
Tsuda, Sakae
影响因子：
3.8
作者：
Maillo, Veronica;de Frutos, Celia;Rizos, Dimitrios
通讯作者：
Rizos, Dimitrios
DOI：
10.1530/jrf.0.0810023
发表时间：
1987-09-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
GANDOLFI, F;MOOR, RM
通讯作者：
MOOR, RM