A biochemical characterization of the DNA binding activity of the response regulator VicR from Streptococcus mutans.
A biochemical characterization of the DNA binding activity of the response regulator VicR from Streptococcus mutans.
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DOI：
10.1371/journal.pone.0108027
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Goodman SD
中科院分区：
文献类型：
--
作者：
Ayala E;Downey JS;Mashburn-Warren L;Senadheera DB;Cvitkovitch DG;Goodman SD
双组分系统（TCS）在细菌中普遍存在，是自然界中最优雅和有效的传感系统之一。它们允许对迅速变化的环境条件作出有效的适应性反应。在这项研究中，我们研究了变形链球菌蛋白VicR的生化特性，这是VicRK TCS的一部分，是一种重要的反应调节剂。我们剖析了VicR在其磷酸化和非磷酸化形式的识别序列的DNA结合要求。通过这样做，我们能够预测S中VicR调节子的扩展。变异人随着越来越多的细菌迅速对最后的抗生素产生耐药性，VicRK等TCS为一类新的抗菌药物提供了有希望的靶标。
Two-component systems (TCSs) are ubiquitous among bacteria and are among the most elegant and effective sensing systems in nature. They allow for efficient adaptive responses to rapidly changing environmental conditions. In this study, we investigated the biochemical characteristics of the Streptococcus mutans protein VicR, an essential response regulator that is part of the VicRK TCS. We dissected the DNA binding requirements of the recognition sequences for VicR in its phosphorylated and unphosphorylated forms. In doing so, we were able to make predictions for the expansion of the VicR regulon within S. mutans. With the ever increasing number of bacteria that are rapidly becoming resistant to even the antibiotics of last resort, TCSs such as the VicRK provide promising targets for a new class of antimicrobials.
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