Chitosan coated carbon fiber microelectrode for selective in vivo detection of neurotransmitters in live zebrafish embryos.
Chitosan coated carbon fiber microelectrode for selective in vivo detection of neurotransmitters in live zebrafish embryos.
复制标题
DOI：
10.1016/j.aca.2011.03.057
复制
发表时间：
2011-06-10
影响因子：
6.2
通讯作者：
Andreescu S
中科院分区：
文献类型：
--
作者：
Ozel RE;Wallace KN;Andreescu S
我们报告了用于体内血清素检测的壳聚糖修饰碳纤维微电极的开发。我们发现壳聚糖能够抑制生理水平的抗坏血酸干扰，并有助于选择性和灵敏地检测体内血清素（一种常见的儿茶酚胺神经递质）水平。使用扫描电子显微镜（SEM）和循环伏安法（CV）研究微电极表面上壳聚糖的存在。使用微分脉冲伏安法（DPV）对电极进行表征。 n=6 电极的检测限为 1.6 nM 血清素，灵敏度为 5.12 nA/μM，线性范围为 2 至 100 nM，重现性为 6.5%。壳聚糖修饰的微电极在存在生理水平的抗坏血酸的情况下选择性地测量血清素。进行体内测量以测量活胚胎斑马鱼肠道中血清素的浓度。该传感器可量化体内肠道血清素水平，同时成功抑制抗坏血酸干扰。我们证明，壳聚糖可以作为 Nafion 的替代品，作为一种有效的涂层来抑制碳纤维微电极上抗坏血酸的干扰，并且壳聚糖修饰的微电极是体内监测神经递质水平变化的可靠工具。
We report the development of a chitosan modified carbon fiber microelectrode for in vivo detection of serotonin. We find that chitosan has the ability to reject physiological levels of ascorbic acid interferences and facilitate selective and sensitive detection of in vivo levels of serotonin, a common catecholamine neurotransmitter. Presence of chitosan on the microelectrode surface was investigated using scanning electron microscopy (SEM) and cyclic voltammetry (CV). The electrode was characterized using differential pulse voltammetry (DPV). A detection limit of 1.6 nM serotonin with a sensitivity of 5.12 nA/µM, a linear range from 2 to 100 nM and a reproducibility of 6.5 % for n=6 electrodes were obtained. Chitosan modified microelectrodes selectively measure serotonin in presence of physiological levels of ascorbic acid. In vivo measurements were performed to measure concentration of serotonin in the live embryonic zebrafish intestine. The sensor quantifies in vivo intestinal levels of serotonin while successfully rejecting ascorbic acid interferences. We demonstrate that chitosan can be used as an effective coating to reject ascorbic acid interferences at carbon fiber microelectrodes, as an alternative to Nafion, and that chitosan modified microelectrodes are reliable tools for in vivo monitoring of changes in neurotransmitter levels.
登录
查看更多内容
影响因子：
6.2
作者：
Jiang, XH;Lin, XQ
通讯作者：
Lin, XQ
影响因子：
7.4
作者：
Njagi, John;Chernov, Mykyta M.;Andreescu, Silvana
通讯作者：
Andreescu, Silvana
影响因子：
2.8
作者：
Pratuangdejkul, J;Nosoongnoen, W;Manivet, P
通讯作者：
Manivet, P
影响因子：
4.2
作者：
Swamy, B. E. Kumara;Venton, B. Jill
通讯作者：
Venton, B. Jill
影响因子：
7.4
作者：
Venton, BJ;Wightman, RM
通讯作者：
Wightman, RM