Transcriptome Analysis on Hepatopancreas Reveals the Metabolic Dysregulation Caused by Vibrio parahaemolyticus Infection in Litopenaeus vannamei.
Transcriptome Analysis on Hepatopancreas Reveals the Metabolic Dysregulation Caused by Vibrio parahaemolyticus Infection in Litopenaeus vannamei.
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急性肝胰腺坏死病(AHPND)是阻碍对虾养殖业发展的致命性疾病。主要由副溶血弧菌引起。为了进一步了解虾对AHPND的抗性机制并培育抗病亲虾，转录组分析已被广泛用于研究对虾对副溶血性弧菌感染的免疫反应，并报道了许多针对该病原体的免疫相关基因。然而，很少有研究关注宿主代谢与弧菌感染之间的关系。在本研究中，我们对副溶血性弧菌感染后不同时间的虾肝胰腺进行了比较转录组分析。我们发现感染后虾肝胰腺中与代谢相关的几个过程和途径显着上调。这些结果表明虾的新陈代谢在应对弧菌感染中发挥着重要作用。这些数据为虾类养殖抗病策略的制定提供了新的视角。急性肝胰腺坏死病（AHPND）导致对虾大量死亡，给水产养殖带来巨大的经济损失。副溶血弧菌（VPAHPND）携带编码与光杆菌昆虫相关（Pir）毒素同源的二元毒素的质粒，是引起该病的主要病原体之一。先前的研究报道了虾的许多免疫相关基因对这种致病细菌的反应。然而，迄今为止，很少有研究关注 VPAHPND 感染后凡纳滨对虾的代谢变化。本研究对 VPAHPND 感染期间不同时间的虾肝胰腺进行了比较转录组分析。对感染过程中差异表达基因（DEG）的功能分析表明，VPAHPND感染后肝胰腺中与葡萄糖、能量和氨基酸代谢以及核酸合成相关的通路发生了明显改变。此外，调节代谢过程的3条信号通路，包括HIF-1信号通路、PI3K-Akt信号通路和NF-KappaB信号通路也发生了显着变化。总的来说，这些数据揭示了宿主代谢过程与弧菌感染之间的密切关系。这些信息将丰富我们对虾和弧菌之间相互作用机制的理解。
Acute hepatopancreas necrosis disease (AHPND) is a lethal disease which hinders the development of shrimp aquaculture. It is mainly caused by Vibrio parahaemolyticus. In order to learn more about the mechanism of resistance to AHPND and breed the disease resistant broodstocks, transcriptome analysis has been widely used to study the immune responses of shrimp to Vibrio parahaemolyticus infection, and many immune-related genes have been reported in response to the pathogen. However, few studies have focused on the relationship between the host metabolism and Vibrio infection. In this study, we performed a comparative transcriptomic analysis on the hepatopancreas of shrimp at different times after V. parahaemolyticus infection. We found that several processes and pathways related to metabolism were significantly upregulated in shrimp hepatopancreas after infection. These results indicate that the metabolism of shrimp plays an important role in response to Vibrio infection. The data provide a new perspective for the development of disease-resistant strategies in shrimp aquaculture. Acute hepatopancreas necrosis disease (AHPND) has caused massive deaths of shrimp and has led to huge economic losses in aquaculture. Vibrio parahaemolyticus (VPAHPND) carrying a plasmid encoding binary toxins homologous to the photorhabdus insect-related (Pir) toxins is one of the main pathogens causing this disease. Previous studies have reported many immune-related genes of shrimp in response to this pathogenic bacteria. However, few studies have so far focused on the metabolic changes in Litopenaeus vannamei upon VPAHPND infection. In the present study, comparative transcriptomic analysis was performed on the hepatopancreas of shrimp at different times during VPAHPND infection. Functional analyses on the differentially expressed genes (DEGs) during infection showed that pathways related to glucose, energy and amino acid metabolism, as well as nucleic acid synthesis, were obviously changed in the hepatopancreas after VPAHPND infection. Additionally, three signaling pathways, which could regulate metabolic processes, including HIF-1 signaling pathway, PI3K-Akt signaling pathway and NF-KappaB signaling pathway, also changed significantly. Collectively, these data reveal a close relationship between host metabolism processes and Vibrio infection. The information will enrich our understanding of the interaction mechanism between the shrimp and Vibrio.
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影响因子：
5.2
作者：
Kumar R;Tung TC;Ng TH;Chang CC;Chen YL;Chen YM;Lin SS;Wang HC
通讯作者：
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影响因子：
7.3
作者：
Mai HN;Caro LFA;Cruz-Flores R;White BN;Dhar AK
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DOI：
10.1128/genomea.01395-15
发表时间：
2015-12-03
期刊：
Genome announcements
影响因子：
--
作者：
Liu L;Xiao J;Xia X;Pan Y;Yan S;Wang Y
通讯作者：
Wang Y
影响因子：
1.4
作者：
Loc Tran;Nunan, Linda;Lightner, Donald V.
通讯作者：
Lightner, Donald V.
影响因子：
6.4
作者：
Karniely S;Weekes MP;Antrobus R;Rorbach J;van Haute L;Umrania Y;Smith DL;Stanton RJ;Minczuk M;Lehner PJ;Sinclair JH
通讯作者：
Sinclair JH