Histological and Biochemical Evaluation of Urethral Scar following Three Different Hypospadias Repairs: An Experimental Study in Rabbits
Histological and Biochemical Evaluation of Urethral Scar following Three Different Hypospadias Repairs: An Experimental Study in Rabbits
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三种不同尿道下裂修复后尿道疤痕的组织学和生化评价：兔实验研究
DOI：
10.1055/s-0037-1605347
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发表时间：
2017-08
影响因子：
1.8
通讯作者：
魏光辉
中科院分区：
文献类型：
--
作者：
谭晓辉;刘星;龙春兰;张德迎;林涛;何大维;魏光辉
尿道成形术已被应用于尿道下裂的外科治疗。神经尿道狭窄是最常见的术后并发症之一，通常需要再次手术。材料与方法通过尿道下裂样缺损和急性修复，建立新西兰白兔尿道下裂模型。24只动物随机分为三组：管状切开板尿道成形术组（TIPU） 8只、基于肉周的皮瓣尿道成形术组（Mathieu） 8只、覆盖岛状皮瓣尿道成形术组（onlay） 8只，均立即行相应手术重建尿道。分别于术后第5天、第2周、第6周、第3个月处死。采集各组大鼠阴茎组织进行组织学和生化检测，检测各组大鼠转化生长因子β1 （TGF-β1）和α-平滑肌肌动蛋白（α-SMactin）的表达情况。结果所有家兔均顺利手术。Mathieu组和onlay组胶原蛋白含量均高于TIPU组（p< 0.05）。生化分析显示，术后2周、6周，TIPU组TGF-β1表达较其他两组明显降低（p< 0.01）。α-SMactin在术后6周和3个月的表达模式相似（p< 0.01）。结论经TIPU修复后的神经系统基本恢复正常解剖结构，瘢痕形成较其他两组明显。因此，TIPU被认为是尿道下裂修复较为合理的入路。成纤维细胞的活性长期升高，这可能是尿道下裂修复后尿道狭窄的发病机制。
IntroductionSeveral urethroplasties have been employed in the surgical treatment of hypospadias. Neourethral strictures are among the most common postoperative complications that often require reoperation.Materials and MethodsWe created a hypospadias model in New Zealand white male rabbits through a hypospadias-like defect and acute repair. A total of 24 animals were randomly allocated into three groups: tubularized incised-plate urethroplasty (TIPU) group (8), perimeatal-based flap urethroplasty (Mathieu) group (8), onlay island flap urethroplasty (onlay) group (8), and corresponding surgical procedures were immediately performed to reconstruct neourethra. The rabbits were killed postoperatively at 5 days, 2 weeks, 6 weeks, and 3 months, respectively. The penile tissue was harvested for histological and biochemical investigations to evaluate the expressions of transforming growth factor β1 (TGF-β1) and α-smooth muscle actin (α-SMactin) in all groups.ResultsAll rabbits were operated on uneventfully. The amount of collagen content was increased in the Mathieu and onlay groups than in the TIPU group (p< 0.05). Biochemical analysis showed that the expression of TGF-β1 in the TIPU group was decreased compared with the two other groups at 2 or 6 weeks postoperatively (p< 0.01). The expression pattern regarding α-SMactin was similar at 6 weeks or 3 months postoperatively (p< 0.01).ConclusionThe neourethra repaired by TIPU was practically resumed to normal anatomy and scarring was less apparent than the two other groups. Therefore, TIPU is considered as a relatively rational approach for hypospadias repair. The activity of fibroblasts has been increased in the long term, which may be the pathogenesis of neourethral stricture following hypospadias repair.
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影响因子：
56.9
作者：
MUSTOE, TA;PIERCE, GF;DEUEL, TF
通讯作者：
DEUEL, TF
影响因子：
2
作者：
Lalla, Marianna;Riis, Carsten;Jorgensen, Troels Munch
通讯作者：
Jorgensen, Troels Munch
DOI：
10.1016/s0022-5347(17)35571-4
发表时间：
1993-08
期刊：
The Journal of urology
影响因子：
--
作者：
L. Baskin;P. Howard;E. Macarak
通讯作者：
L. Baskin;P. Howard;E. Macarak
影响因子：
2.6
作者：
Mizuno, K;Hayashi, Y;Kohri, K
通讯作者：
Kohri, K
影响因子：
--
作者：
F. Taneli;C. Ulman;A. Genç;O. Yilmaz;C. Taneli
通讯作者：
F. Taneli;C. Ulman;A. Genç;O. Yilmaz;C. Taneli