Immunocytochemical localization of oestrogen receptors in the endometrium of the ewe.
Immunocytochemical localization of oestrogen receptors in the endometrium of the ewe.
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母羊子宫内膜中雌激素受体的免疫细胞化学定位。
DOI：
10.1071/rd9910321
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
J. Findlay
中科院分区：
文献类型：
--
作者：
RA Cherny;La Salamonsen;J. Findlay
雌激素受体（ER）的单克隆抗体的免疫细胞化学被用来本地化ER的周期和怀孕的Corriedale母羊子宫内膜的部分。使用周期第4、10、14、15、16和17天（第0天=发情开始）和妊娠第15天的代表性组织。雌激素受体定位也进行了检查，在组织中获得卵巢切除（ESTX）母羊和皮下植入含有雌激素，孕激素，或雌激素和孕激素。结果：ER在子宫内膜中的分布呈阳性，在子宫内膜间质中的分布呈阴性。染色强度根据细胞类型、周期阶段、类固醇治疗和妊娠而变化。在内皮细胞中未观察到染色。在所有病例中，ER定位于阳性细胞的细胞核内。一般来说，ER水平在第4天较高，到第10天下降到可忽略的值（对应于孕酮峰值），但在泪阜子宫内膜的深层基质中除外。阳性染色在第13天的肉阜基质细胞和第14天的肉阜间组织的腔上皮中再次出现。在第15天，种阜组织达到峰值强度，在第16天，种阜间组织达到峰值强度。卵巢切除术并没有导致ER水平的整体下降，而雌激素和孕激素治疗的效果取决于细胞类型。预后不抑制整体ER。在妊娠第15天的组织中，除了肉阜的深层基质外，在所有隔室中均检测不到ER，这表明可能是胚胎起源的孕酮以外的因素。观察到个别细胞类型显示不同的敏感性，雌激素和孕激素的表达方面是一致的细胞-细胞相互作用的作用，作为调节剂的细胞反应类固醇通过发情周期和怀孕。
Immunocytochemistry with monoclonal antibodies to the oestrogen receptor (ER) was used to localize ERs in sections of endometrium obtained from cycling and pregnant Corriedale ewes. Representative tissue from Days 4, 10, 14, 15, 16 and 17 of the cycle (Day 0 = onset of oestrus) and Day 15 of pregnancy was used. ER localization was also examined in tissue obtained from ovariectomized (ovex) ewes with and without subcutaneous implants containing oestrogen, progesterone, or oestrogen and progesterone. ER distribution was examined in caruncular endometrium and intercaruncular endometrium. Staining intensity varied according to cell type, stage of the cycle, steroid treatment and pregnancy. No staining was observed in endothelial cells. In all cases, ER was localized within the nuclei of positive cells. Generally, ER levels were high on Day 4 and declined to negligible values by Day 10 (corresponding to peak progesterone values) except in the deep stroma of caruncular endometrium. Positive staining reappeared in stromal cells of caruncles on Day 13 and in the luminal epithelium of intercaruncular tissue on Day 14. Peak intensity was reached on Day 15 for caruncular tissue and Day 16 for intercaruncular tissue. Ovariectomy did not cause an overall reduction in ER levels, whereas treatment with oestrogen and progesterone had variable effects depending on cell type. Progesterone did not suppress overall ER. In Day 15 pregnant tissue, ER was undetectable in all compartments except deep stroma of caruncles, indicating that factors other than progesterone, perhaps embryonic in origin, were responsible. The observation that individual cell types display differential sensitivities to oestrogen and progesterone as regards their expression of ER is consistent with the role of cell-cell interactions as modulators of cellular response to steroids through the oestrous cycle and in pregnancy.
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DOI：
10.1097/00006254-198510000-00017
发表时间：
1984-11
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
M. Press;N. Nousek-Goebl;W. King;A. Herbst;G. Greene
通讯作者：
M. Press;N. Nousek-Goebl;W. King;A. Herbst;G. Greene
影响因子：
3.6
作者：
BRENNER, RM;WEST, NB;MCCLELLAN, MC
通讯作者：
MCCLELLAN, MC
双免疫荧光法检测兔子宫雌、孕激素受体的差异分布。
影响因子：
4.8
作者：
Zaino,RJ;Clarke,CL;Feil,PD;Satyaswaroop,PG
通讯作者：
Satyaswaroop,PG
影响因子：
5.8
作者：
LESSEY, BA;KILLAM, AP;MCCARTY, KS
通讯作者：
MCCARTY, KS
黄体酮停药后，绵羊子宫中核雌激素受体和催产素受体迅速恢复。
DOI：
10.1016/0022-4731(85)90272-9
发表时间：
1985
期刊：
Journal of steroid biochemistry
影响因子：
--
作者：
Leavitt,WW;Okulicz,WC;McCracken,JA;Schramm,W;RobidouxJr,WF
通讯作者：
RobidouxJr,WF