Expression of foot-and-mouth disease virus capsid proteins in silkworm-baculovirus expression system and its utilization as a subunit vaccine.
Expression of foot-and-mouth disease virus capsid proteins in silkworm-baculovirus expression system and its utilization as a subunit vaccine.
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DOI：
10.1371/journal.pone.0002273
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发表时间：
2008-05-28
期刊：
影响因子：
3.7
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Li Z;Yi Y;Yin X;Zhang Z;Liu J
口蹄疫（Foot-and-mouth disease，FMD）是一种高度传染性的家畜疾病，对易感的偶蹄类动物造成严重的经济损失。传统的灭活疫苗虽然已被证明是有效的，但由于FMDV灭活不完全或活病毒从疫苗生产车间逃逸，可能导致新的口蹄疫暴发。因此，迫切需要开发一种比传统疫苗更安全、更有效、更经济的新型口蹄疫疫苗。本研究成功构建了含有口蹄疫病毒亚洲株1/HNK/CHA/05 P1-2A和3C蛋白酶编码区的重组家蚕杆状病毒Bm-P1 - 2A 3C。用间接免疫荧光法和ELISA法检测Bm-P12 A3 C的表达。将家蚕表达产物稀释至30倍，作为抗原免疫牛。在所有接种动物中诱导特异性抗体。用同源毒攻毒后，5只动物中有4只完全受到保护，其余1只动物的临床症状减轻并延迟。此外，还进行了PD 50（50%牛保护剂量）试验，以评估亚单位疫苗的牛效价。结果表明，该亚单位疫苗每剂可获得6.34个PD_（50）。结果表明，该策略可用于口蹄疫病毒亚单位疫苗的研制。
Foot-and-mouth disease (FMD) is a highly contagious disease of livestock that causes severe economic loss in susceptible cloven-hoofed animals. Although the traditional inactivated vaccine has been proved effective, it may lead to a new outbreak of FMD because of either incomplete inactivation of FMDV or the escape of live virus from vaccine production workshop. Thus, it is urgent to develop a novel FMDV vaccine that is safer, more effective and more economical than traditional vaccines. A recombinant silkworm baculovirus Bm-P12A3C which contained the intact P1-2A and 3C protease coding regions of FMDV Asia 1/HNK/CHA/05 was developed. Indirect immunofluorescence test and sandwich-ELISA were used to verify that Bm-P12A3C could express the target cassette. Expression products from silkworm were diluted to 30 folds and used as antigen to immunize cattle. Specific antibody was induced in all vaccinated animals. After challenge with virulent homologous virus, four of the five animals were completely protected, and clinical symptoms were alleviated and delayed in the remaining one. Furthermore, a PD50 (50% bovine protective dose) test was performed to assess the bovine potency of the subunit vaccine. The result showed the subunit vaccine could achieve 6.34 PD50 per dose. The results suggest that this strategy might be used to develop the new subunit FMDV vaccine.
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