Cloacibacillus porcorum sp. nov., a mucin-degrading bacterium from the swine intestinal tract and emended description of the genus Cloacibacillus.
Cloacibacillus porcorum sp. nov., a mucin-degrading bacterium from the swine intestinal tract and emended description of the genus Cloacibacillus.
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DOI：
10.1099/ijs.0.044719-0
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发表时间：
2013-06
影响因子：
2.8
通讯作者：
Stanton TB
中科院分区：
文献类型：
--
作者：
Looft T;Levine UY;Stanton TB
从猪肠道中分离到一株新的厌氧、嗜中温、氨基酸发酵菌CL-84 T。细胞呈弯曲杆状（0.8-1.2×3.5-5.0 µm），染色为革兰氏阴性，不活动，无孢子迹象。菌株CL-84 T在丝氨酸上生长时产生乙酸盐、丙酸盐、甲酸盐和丁酸盐作为代谢终产物。最佳生长发生在39 °C和pH 6.5。菌株CL-84 T的主要细胞脂肪酸为iso-C15：0、iso-C15：0 3-OH、iso-C17：0和C16：0。 菌株CL-84 T的DNA G+C含量为55.1mol%。16 S rRNA基因序列分析表明，菌株CL-84 T与合生元科合生元门（Synergistetes）的特征属有90- 95%的相似性。 系统发育分析表明，菌株CL-84 T与埃夫里梭芽孢杆菌（Cloacibacillusevryensis）有亲缘关系，但与埃夫里梭芽孢杆菌有明显区别。基于这些发现，我们提出菌株CL-84 T代表了一个新的物种的Cloacibacillus属。我们进一步建议将该种命名为Porcorum Cloacibacillus sp. nov.。模式菌株为CL-84 T（= DSM 25858 T = CCUG 62631 T）。 提供了一个修订的描述的属Cloacibacillus。
A novel anaerobic, mesophilic, amino-acid-fermenting bacterium, designated strain CL-84T, was isolated from the swine intestinal tract on mucin-based media. Cells were curved rods (0.8–1.2×3.5–5.0 µm), stained Gram-negative and were non-motile with no evidence of spores. Strain CL-84T produced acetate, propionate, formate and butyrate as the end products of metabolism when grown on serine. Optimum growth occurred at 39 °C and pH 6.5. The major cellular fatty acids were iso-C15 : 0, iso-C15 : 0 3-OH, iso-C17 : 0 and C16 : 0, distinguishing strain CL-84T from closely related species. The DNA G+C content of strain CL-84T was 55.1 mol%. 16S rRNA gene sequence analysis showed that strain CL-84T shared 90–95 % similarity with characterized genera within the phylum Synergistetes, family Synergistaceae. Phylogenetic analysis showed that strain CL-84T was related to, but distinct from, Cloacibacillus evryensis. Based on these findings, we propose that strain CL-84T represents a novel species of the genus Cloacibacillus. We further propose the name Cloacibacillus porcorum sp. nov. be designated for this species. The type strain is CL-84T ( = DSM 25858T = CCUG 62631T). An emended description of the genus Cloacibacillus is provided.
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影响因子：
30.3
作者：
Martens EC;Chiang HC;Gordon JI
通讯作者：
Gordon JI
影响因子：
14.9
作者：
Ludwig, W;Strunk, O;Schleifer, KH
通讯作者：
Schleifer, KH
影响因子：
14.9
作者：
Pruesse E;Quast C;Knittel K;Fuchs BM;Ludwig W;Peplies J;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
24.5
作者：
FINNIE, IA;DWARAKANATH, AD;RHODES, JM
通讯作者：
RHODES, JM
DOI：
10.1111/j.1432-1033.1981.tb06404.x
发表时间：
1981-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
BUCKEL, W;DORN, U;SEMMLER, R
通讯作者：
SEMMLER, R