Exploring the diversity of Gardnerella vaginalis in the genitourinary tract microbiota of monogamous couples through subtle nucleotide variation.
Exploring the diversity of Gardnerella vaginalis in the genitourinary tract microbiota of monogamous couples through subtle nucleotide variation.
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DOI：
10.1371/journal.pone.0026732
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Ferris MJ
中科院分区：
文献类型：
--
作者：
Eren AM;Zozaya M;Taylor CM;Dowd SE;Martin DH;Ferris MJ
细菌性阴道病（BV）是一种原因不明的神秘疾病，影响了很大比例的妇女。BV女性的阴道微生物群与严重的后遗症有关，包括异常妊娠。BV的病因尚未完全清楚，但已提出它是可传播的，G。迷走神经病可能是一种病因。利用酶法对G. vaginocarcinoma生物型，以及最近的基因组比较G。从有症状和无症状的妇女中分离到的G.迷走病毒株可能在BV的发病机制中起关键作用。探讨G.为了分析65,710 G中的低水平序列变异，我们开发了一种基于Shannon熵的方法来分析65,710 G的低水平序列变异。从53名一夫一妻制妇女的阴道样本和她们男性伴侣的尿道和阴茎皮肤样本中PCR扩增的阴道炎16 S rRNA基因片段。我们观察到G.研究人员发现了具有总共46个独特序列变体（寡聚型）的迷走神经基因序列，并且还发现这些寡聚型在性伴侣之间具有很强的相关性。尽管在UniFrac分析中，革兰氏染色确定的正常和一些革兰氏染色确定的中间寡聚型谱聚集在一起，但没有单一的G。发现阴道炎寡型对BV或正常阴道样品具有特异性。本研究描述了一种新的研究G.在低水平的分类歧视的vagulary多样性。这一发现支持了基于培养的研究，即性伴侣携带相同的G。流浪汉这项研究还强调了这样一个事实，即16 S rRNA基因内的一些可重复的核苷酸变异可以揭示16 S rRNA数据的属级或种级分类所遗漏的临床或流行病学关联。
Bacterial vaginosis (BV) is an enigmatic disease of unknown origin that affects a large percentage of women. The vaginal microbiota of women with BV is associated with serious sequelae, including abnormal pregnancies. The etiology of BV is not fully understood, however, it has been suggested that it is transmissible, and that G. vaginalis may be an etiological agent. Studies using enzymatic assays to define G. vaginalis biotypes, as well as more recent genomic comparisons of G. vaginalis isolates from symptomatic and asymptomatic women, suggest that particular G. vaginalis strains may play a key role in the pathogenesis of BV. To explore G. vaginalis diversity, distribution and sexual transmission, we developed a Shannon entropy-based method to analyze low-level sequence variation in 65,710 G. vaginalis 16S rRNA gene segments that were PCR-amplified from vaginal samples of 53 monogamous women and from urethral and penile skin samples of their male partners. We observed a high degree of low-level diversity among G. vaginalis sequences with a total of 46 unique sequence variants (oligotypes), and also found strong correlations of these oligotypes between sexual partners. Even though Gram stain-defined normal and some Gram stain-defined intermediate oligotype profiles clustered together in UniFrac analysis, no single G. vaginalis oligotype was found to be specific to BV or normal vaginal samples. This study describes a novel method for investigating G. vaginalis diversity at a low level of taxonomic discrimination. The findings support cultivation-based studies that indicate sexual partners harbor the same strains of G. vaginalis. This study also highlights the fact that a few, reproducible nucleotide variations within the 16S rRNA gene can reveal clinical or epidemiological associations that would be missed by genus-level or species-level categorization of 16S rRNA data.
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影响因子：
12.3
作者：
Huse SM;Huber JA;Morrison HG;Sogin ML;Welch DM
通讯作者：
Welch DM
影响因子：
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作者：
De Backer, Ellen;Verhelst, Rita;Vaneechoutte, Mario
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Vaneechoutte, Mario
影响因子：
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作者：
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Jefferson, Kimberly K.
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通讯作者：
Vaneechoutte, Mario
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3.1
作者：
Marrazzo JM;Martin DH;Watts DH;Schulte J;Sobel JD;Hillier SL;Deal C;Fredricks DN
通讯作者：
Fredricks DN