Modification of Salmonella Typhimurium motility by the probiotic yeast strain Saccharomyces boulardii.
Modification of Salmonella Typhimurium motility by the probiotic yeast strain Saccharomyces boulardii.
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DOI：
10.1371/journal.pone.0033796
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Czerucka D
中科院分区：
文献类型：
--
作者：
Pontier-Bres R;Prodon F;Munro P;Rampal P;Lemichez E;Peyron JF;Czerucka D
运动性是肠沙门氏菌鼠伤寒血清型 (ST) 发病机制的重要组成部分，使细菌能够进入适当的生态位，穿过粘液层并侵入肠上皮。在体外，鞭毛相关运动与 ST 的侵袭特性密切相关。益生菌布拉酵母菌 BIOCODEX (S.b-B) 被广泛用于预防和治疗由细菌或抗生素引起的腹泻疾病。在沙门氏菌感染的情况下，S.b-B 已被证明可以减少 T84 结肠细胞系的 ST 侵袭。本研究旨在调查 S.b-B 对 ST 运动的影响。实验在单独被沙门氏菌菌株 1344 或在 S.b-B 存在的情况下感染的人结肠 T84 细胞上进行。通过延时视频显微镜记录沙门氏菌的运动性。接下来，进行手动跟踪来分析细菌动态（MTrackJ插件，NIH image J软件）。这表明细菌运动的速度在 S.b-B 存在的情况下发生了改变。在 S.b-B 存在的情况下，单独与 T84 一起孵育的沙门氏菌的中值曲线速度 (CLV) 从 43.3 µm/秒降至 31.2 µm/秒。轨迹线性 (TL) 的测量显示出类似的趋势：S.b-B 使具有线性道 (LT) 的细菌数量减少 15%，使具有旋转道 (RT) 的细菌数量增加 22%。通过研究非运动鞭毛缺陷的 ST 菌株，进一步建立了 ST 运动与侵袭之间的相关性。事实上，该菌株以 0.5 µm/秒的 CLV 移动，表现出大部分 RT 和侵袭特性显着下降。重要的是，我们发现 S.b-B 改变了致病菌株 SL1344 的运动性，并显着减少了该菌株对 T84 细胞的侵袭。这项研究表明，S.b-B 改变了沙门氏菌的运动和轨迹，这可能是改变沙门氏菌入侵的原因。
Motility is an important component of Salmonella enterica serovar Typhimurium (ST) pathogenesis allowing the bacteria to move into appropriate niches, across the mucus layer and invade the intestinal epithelium. In vitro, flagellum-associated motility is closely related to the invasive properties of ST. The probiotic yeast Saccharomyces boulardii BIOCODEX (S.b-B) is widely prescribed for the prophylaxis and treatment of diarrheal diseases caused by bacteria or antibiotics. In case of Salmonella infection, S.b-B has been shown to decrease ST invasion of T84 colon cell line. The present study was designed to investigate the impact of S.b-B on ST motility. Experiments were performed on human colonic T84 cells infected by the Salmonella strain 1344 alone or in the presence of S.b-B. The motility of Salmonella was recorded by time-lapse video microscopy. Next, a manual tracking was performed to analyze bacteria dynamics (MTrackJ plugin, NIH image J software). This revealed that the speed of bacterial movement was modified in the presence of S.b-B. The median curvilinear velocity (CLV) of Salmonella incubated alone with T84 decreased from 43.3 µm/sec to 31.2 µm/sec in the presence of S.b-B. Measurement of track linearity (TL) showed similar trends: S.b-B decreased by 15% the number of bacteria with linear tract (LT) and increased by 22% the number of bacteria with rotator tract (RT). Correlation between ST motility and invasion was further established by studying a non-motile flagella-deficient ST strain. Indeed this strain that moved with a CLV of 0.5 µm/sec, presented a majority of RT and a significant decrease in invasion properties. Importantly, we show that S.b-B modified the motility of the pathogenic strain SL1344 and significantly decreased invasion of T84 cells by this strain. This study reveals that S.b-B modifies Salmonella's motility and trajectory which may account for the modification of Salmonella's invasion.
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影响因子：
3.7
作者：
Martins FS;Dalmasso G;Arantes RM;Doye A;Lemichez E;Lagadec P;Imbert V;Peyron JF;Rampal P;Nicoli JR;Czerucka D
通讯作者：
Czerucka D
影响因子：
3.8
作者：
KHORAMIANFALSAFI, T;HARAYAMA, S;PECHERE, JC
通讯作者：
PECHERE, JC
影响因子：
29.4
作者：
POTHOULAKIS, C;KELLY, CP;LAMONT, JT
通讯作者：
LAMONT, JT
影响因子：
3.1
作者：
JONES, BD;LEE, CA;FALKOW, S
通讯作者：
FALKOW, S
影响因子：
3.4
作者：
Karim, QN;Logan, RPH;Worku, ML
通讯作者：
Worku, ML