The seed region of a small RNA drives the controlled destruction of the target mRNA by the endoribonuclease RNase E.
The seed region of a small RNA drives the controlled destruction of the target mRNA by the endoribonuclease RNase E.
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DOI：
10.1016/j.molcel.2012.07.015
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发表时间：
2012-09-28
期刊：
影响因子：
16
通讯作者：
Luisi, Ben F.
中科院分区：
文献类型：
--
作者：
Bandyra, Katarzyna J.;Said, Nelly;Pfeiffer, Verena;Gorna, Maria W.;Vogel, Joerg;Luisi, Ben F.
细菌中的许多小的非编码RNA（sRNA）调节靶mRNA的翻译起始和降解速率，它们通过RNA伴侣Hfq促进的碱基配对来识别靶mRNA。最近的证据表明Hfq、sRNA和mRNA的三元复合物引导核糖核酸内切酶RNase E启动两种RNA的周转。我们发现，sRNA不仅引导RNase E到靶RNA中的确定位点，而且还通过在同源配对“种子”的5′端呈现一个单磷酸基团来变构激活酶。此外，在没有靶点的情况下，5′-单磷酸使sRNA种子区容易受到RNA酶E的攻击，而Hfq对此没有保护作用。这些结果表明，sRNA种子区的化学特征和配对状态可能有助于“校对”识别和激活mRNA切割，作为涉及RNA、Hfq和RNase E合作的动态过程的一部分。小RNA-mRNA双链体可以募集单链特异性核糖核酸内切酶RNase E，sRNA可以引导和变构激活RNase E，启动靶mRNA降解，变构信号是sRNA 5'端的一个单磷酸基团，5'端单磷酸可能有助于sRNA作用的校正。
Numerous small non-coding RNAs (sRNAs) in bacteria modulate rates of translation initiation and degradation of target mRNAs, which they recognize through base-pairing facilitated by the RNA chaperone Hfq. Recent evidence indicates that the ternary complex of Hfq, sRNA and mRNA guides endoribonuclease RNase E to initiate turnover of both the RNAs. We show that a sRNA not only guides RNase E to a defined site in a target RNA, but also allosterically activates the enzyme by presenting a monophosphate group at the 5′-end of the cognate-pairing “seed.” Moreover, in the absence of the target the 5′-monophosphate makes the sRNA seed region vulnerable to an attack by RNase E against which Hfq confers no protection. These results suggest that the chemical signature and pairing status of the sRNA seed region may help to both ‘proofread’ recognition and activate mRNA cleavage, as part of a dynamic process involving cooperation of RNA, Hfq and RNase E. ► Small RNA-mRNA duplex can recruit single-strand specific endoribonuclease RNase E ► sRNA can guide and allosterically activate RNase E to initiate target mRNA degradation ► The allosteric signal is a monophosphate group on the 5' end of the sRNA ► The 5' monophosphate may contribute to proofreading of sRNA action
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