Verticillium dahliae secreted protein Vd424Y is required for full virulence, targets the nucleus of plant cells, and induces cell death.
Verticillium dahliae secreted protein Vd424Y is required for full virulence, targets the nucleus of plant cells, and induces cell death.
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大丽轮枝菌分泌蛋白Vd424Y是完全毒力所必需的，它靶向植物细胞核并诱导细胞死亡。
DOI：
10.1111/mpp.13100
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发表时间：
2021-09
影响因子：
4.9
通讯作者：
Ge X
中科院分区：
文献类型：
--
作者：
Liu L;Wang Z;Li J;Wang Y;Yuan J;Zhan J;Wang P;Lin Y;Li F;Ge X
真菌病原体分泌效应蛋白来调节宿主免疫，并能抑制植物中针对定殖的基础防御机制。大丽轮枝菌是一种广泛存在且具有破坏性的土传真菌，可导致植物维管束萎蔫病并降低植物产量。然而，目前对于大丽轮枝菌所分泌的效应蛋白如何发挥作用知之甚少。在本研究中，我们分析并鉴定了大丽轮枝菌分泌组中的34种候选效应蛋白，发现一种糖苷水解酶11家族蛋白Vd424Y在棉花植株被大丽轮枝菌感染的早期阶段高度上调。该蛋白定位于细胞核，其缺失会削弱真菌的毒力。Vd424Y在本氏烟草中的瞬时表达诱导了依赖于BAK1和SOBIR1的细胞死亡，并激活了水杨酸和茉莉酸信号通路。这以依赖于其核定位信号和信号肽的方式增强了其对卵菌辣椒疫霉的抗性。我们的研究结果表明，Vd424Y是一种重要的效应蛋白，它靶向宿主细胞核以调节和激活植物中效应蛋白触发的免疫。
Vd424Y是一种重要的效应蛋白，它靶向宿主细胞核以调节和激活植物中效应蛋白触发的免疫。
Fungal pathogens secrete effector proteins that regulate host immunity and can suppress basal defence mechanisms against colonization in plants. Verticillium dahliae is a widespread and destructive soilborne fungus that can cause vascular wilt disease and reduces plant yields. However, little is currently known about how the effectors secreted by V. dahliae function. In this study, we analysed and identified 34 candidate effectors in the V. dahliae secretome and found that Vd424Y, a glycoside hydrolase family 11 protein, was highly upregulated during the early stages of V. dahliae infection in cotton plants. This protein was located in the nucleus and its deletion compromised the virulence of the fungus. The transient expression of Vd424Y in Nicotiana benthamiana induced BAK1‐ and SOBIR1‐dependent cell death and activated both salicylic acid and jasmonic acid signalling. This enhanced its resistance to the oomycetes Phytophthora capsici in a way that depended on its nuclear localization signal and signal peptides. Our results demonstrate that Vd424Y is an important effector protein targeting the host nucleus to regulate and activate effector‐triggered immunity in plants. Vd424Y is an important effector protein targeting the host nucleus to regulate and activate effector‐triggered immunity in plants.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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