Multiplexed Biosensing of Proteins and Virions with Disposable Plasmonic Assays.
Multiplexed Biosensing of Proteins and Virions with Disposable Plasmonic Assays.
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DOI：
10.1021/acssensors.2c02238
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发表时间：
2023-09-22
期刊：
影响因子：
8.9
通讯作者：
Karimullah, Affar S.
中科院分区：
文献类型：
--
作者：
Wallace, Stephanie;Kartau, Martin;Kakkar, Tarun;Davis, Chris;Szemiel, Agnieszka;Samardzhieva, Iliyana;Vijayakrishnan, Swetha;Cole, Sarah;De Lorenzo, Giuditta;Maillart, Emmanuel;Gautier, Kevin;Lapthorn, Adrian J.;Patel, Arvind H.;Gadegaard, Nikolaj;Kadodwala, Malcolm;Hutchinson, Edward;Karimullah, Affar S.
我们根据个人需求量身定制医疗保健的能力日益增强，这有可能改变临床治疗。然而，多种生物标志物的测量为临床决策提供信息需要快速、有效和负担得起的诊断。慢性病和在更大人群中迅速演变的病原体也使提高诊断能力的需要更加迫切。目前的化学诊断通常在集中的设施中进行，仍然依赖于多个步骤、分子标记和详细分析，导致结果周转时间超过数小时甚至数天。基于侧流装置的快速诊断试剂盒可以快速返回结果，但只能检测少数病原体或标记物。在此，我们提出了使用一次性等离子体与热敏纳米结构作为低成本、无标签的多路光生物传感平台，而不需要当前光学生物传感器通常需要的流系统。我们展示了复杂介质中SARS-CoV-2的检测以及诺如病毒和寨卡病毒的检测，作为用于多种呼吸道病毒鉴别诊断和任何其他新兴诊断需求的高通量单步诊断试剂盒的早期发展里程碑。基于该平台的诊断，我们称之为“一次性等离子体分析”，将适用于近护理点环境中多种病原体或生物标志物的低成本筛查。
Our growing ability to tailor healthcare to the needs of individuals has the potential to transform clinical treatment. However, the measurement of multiple biomarkers to inform clinical decisions requires rapid, effective, and affordable diagnostics. Chronic diseases and rapidly evolving pathogens in a larger population have also escalated the need for improved diagnostic capabilities. Current chemical diagnostics are often performed in centralized facilities and are still dependent on multiple steps, molecular labeling, and detailed analysis, causing the result turnaround time to be over hours and days. Rapid diagnostic kits based on lateral flow devices can return results quickly but are only capable of detecting a handful of pathogens or markers. Herein, we present the use of disposable plasmonics with chiroptical nanostructures as a platform for low-cost, label-free optical biosensing with multiplexing and without the need for flow systems often required in current optical biosensors. We showcase the detection of SARS-CoV-2 in complex media as well as an assay for the Norovirus and Zika virus as an early developmental milestone toward high-throughput, single-step diagnostic kits for differential diagnosis of multiple respiratory viruses and any other emerging diagnostic needs. Diagnostics based on this platform, which we term “disposable plasmonics assays,” would be suitable for low-cost screening of multiple pathogens or biomarkers in a near-point-of-care setting.
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DOI：
10.1099/jgv.0.000535
发表时间：
2016-08
期刊：
The Journal of general virology
影响因子：
--
作者：
Dadonaite B;Vijayakrishnan S;Fodor E;Bhella D;Hutchinson EC
通讯作者：
Hutchinson EC
影响因子：
6.4
作者：
Koczula KM;Gallotta A
通讯作者：
Gallotta A
DOI：
10.1038/s41377-020-00433-1
发表时间：
2020-12-01
期刊：
Light, science & applications
影响因子：
--
作者：
Kakkar T;Keijzer C;Rodier M;Bukharova T;Taliansky M;Love AJ;Milner JJ;Karimullah AS;Barron LD;Gadegaard N;Lapthorn AJ;Kadodwala M
通讯作者：
Kadodwala M
影响因子：
2.1
作者：
AHLERS, M;BLANKENBURG, R;SALESSE, C
通讯作者：
SALESSE, C
影响因子：
15
作者：
Kelly C;Tullius R;Lapthorn AJ;Gadegaard N;Cooke G;Barron LD;Karimullah AS;Rotello VM;Kadodwala M
通讯作者：
Kadodwala M