Disruption of the S41 peptidase gene in mycoplasma mycoides capri impacts proteome profile, H(2)O(2) production, and sensitivity to heat shock.
Disruption of the S41 peptidase gene in mycoplasma mycoides capri impacts proteome profile, H(2)O(2) production, and sensitivity to heat shock.
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DOI：
10.1371/journal.pone.0051345
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Reyes L
中科院分区：
文献类型：
--
作者：
Allam AB;Brown MB;Reyes L
蕈状支原体簇的成员是支原体中毒性最强的，引起世界范围内经济上重要的牛和山羊疾病。簇成员之间的区别表型是降解酪蛋白的能力。MMCP2_0241基因是一种S41肽酶，在丝状支原体丝状亚种中赋予蛋白水解表型。卡普里GM 12.为了确定基因破坏的影响，我们使用差异蛋白质组分析来比较M。蕈状亚种具有缺乏蛋白水解表型的突变体的卡普里野生型。MMCP2_0241的破坏导致改变的表型，使人联想到M.蕈状亚种mycoides SC和微生物的蛋白质组谱有显着的影响。该突变体表现出过氧化氢的产生增加，乳酸脱氢酶活性降低，并增加对热休克的敏感性。
Members of the Mycoplasma mycoides cluster are among the most virulent of the mycoplasmas, causing worldwide economically significant diseases of cattle and goats. A distinguishing phenotype among the members of the cluster is the ability to degrade casein. The MMCAP2_0241 gene, an S41 peptidase, confers the proteolytic phenotype in Mycoplasma mycoides subsp. capri GM12. In order to determine the impact of disruption of the gene, we used differential proteome profiling to compare the M. mycoides subsp. capri wild type with a mutant lacking the proteolytic phenotype. Disruption of MMCAP2_0241 resulted in altered phenotypes reminiscent of M. mycoides subsp. mycoides SC and had significant impacts on the proteome profile of the microbe. The mutant exhibited increased production of hydrogen peroxide, decreased lactate dehydrogenase activity, and increased sensitivity to heat shock.
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影响因子：
14.9
作者：
Dimmer EC;Huntley RP;Alam-Faruque Y;Sawford T;O'Donovan C;Martin MJ;Bely B;Browne P;Mun Chan W;Eberhardt R;Gardner M;Laiho K;Legge D;Magrane M;Pichler K;Poggioli D;Sehra H;Auchincloss A;Axelsen K;Blatter MC;Boutet E;Braconi-Quintaje S;Breuza L;Bridge A;Coudert E;Estreicher A;Famiglietti L;Ferro-Rojas S;Feuermann M;Gos A;Gruaz-Gumowski N;Hinz U;Hulo C;James J;Jimenez S;Jungo F;Keller G;Lemercier P;Lieberherr D;Masson P;Moinat M;Pedruzzi I;Poux S;Rivoire C;Roechert B;Schneider M;Stutz A;Sundaram S;Tognolli M;Bougueleret L;Argoud-Puy G;Cusin I;Duek-Roggli P;Xenarios I;Apweiler R
通讯作者：
Apweiler R
影响因子：
3.1
作者：
Bischof, Daniela F.;Janis, Carole;Frey, Joachim
通讯作者：
Frey, Joachim
影响因子：
2.2
作者：
Khan, LA;Miles, RJ;Nicholas, RAJ
通讯作者：
Nicholas, RAJ
影响因子：
14.9
作者：
Barré, A;de Daruvar, A;Blanchard, A
通讯作者：
Blanchard, A
影响因子：
2.8
作者：
BenMenachem, G;Himmelreich, R;Rottem, S
通讯作者：
Rottem, S