Differential expression of myc, max and RB1 genes in human gliomas and glioma cell lines.
Differential expression of myc, max and RB1 genes in human gliomas and glioma cell lines.
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DOI：
10.1038/bjc.1994.3
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发表时间：
1994-01
影响因子：
8.8
通讯作者：
Kalimo, Hannu
中科院分区：
文献类型：
--
作者：
Hirvonen, Harri E.;Salonen, Reijo;Sandberg, Minna M.;Vuorio, Eero;Vastrik, Imre;Kotilainen, Esa;Kalimo, Hannu
myc原癌基因表达失调与几种人类肿瘤有关。我们分析了c-myc，N-myc，L-myc，max和RB 1 mRNA在一组人脑胶质瘤和胶质瘤细胞系中的表达，并与正常神经细胞的结果进行了比较。max和RB 1基因被包括在研究中，因为它们的蛋白质产物可以与Myc蛋白相互作用，因此被认为是Myc活性的调节剂。几个胶质瘤含有c/L-myc mRNA的水平高于胎儿脑，L-myc主要在II/III级和c-myc在III级胶质瘤。检测到高水平的N-myc表达。在一个小细胞胶质母细胞瘤中，其他五个胶质瘤中的水平较低。相反，胶质瘤细胞系完全缺乏N/L-myc表达。原位杂交显示myc和胶质细胞酸性蛋白（GFAP）mRNA的相互排斥的地形分布，myc表达和增殖活性之间缺乏相关性，max和RB 1 mRNA在大多数肿瘤和细胞系中检测到。神经胶质瘤细胞显示出编码Max蛋白的Max mRNA的有趣的选择性剪接模式，其抑制（Max）或增强（Δ Max）Myc的转化活性。我们的结论是：（1）胶质瘤细胞在体内可能共表达几个myc基因，因此类似于胎儿神经细胞;但（2）培养的胶质瘤细胞只表达c-myc;（3）myc，max和RB 1在胶质瘤细胞中独立调节;（4）在一些胶质瘤细胞中，max mRNA的交替加工导致在正常胎儿脑中未见的delta Max编码mRNA。
Deregulated expression of myc proto-oncogenes is implicated in several human neoplasias. We analysed the expression of c-myc, N-myc, L-myc, max and RB1 mRNAs in a panel of human gliomas and glioma cell lines and compared the findings with normal neural cells. The max and RB1 genes were included in the study because their protein products can interact with the Myc proteins, being thus putative modulators of Myc activity. Several gliomas contained c/L-myc mRNAs at levels higher than those in fetal brain, L-myc predominantly in grade II/III and c-myc in grade III gliomas. High-level N-myc expression was detected. In one small-cell glioblastoma and lower levels in five other gliomas. In contrast, glioma cell lines totally lacked N/L-myc expression. The in situ hybridisations revealed mutually exclusive topographic distribution of myc and glial fibrillary acidic protein (GFAP) mRNAs, and a lack of correlation between myc expression and proliferative activity, max and RB1 mRNAs were detected in most tumours and cell lines. The glioma cells displayed interesting alternative splicing patterns of max mRNAs encoding Max proteins which either suppress (Max) or augment (delta Max) the transforming activity of Myc. We conclude that (1) glioma cells in vivo may coexpress several myc genes, thus resembling fetal neural cells; but (2) cultured glioma cells expression only c-myc; (3) myc, max and RB1 are regulated independently in glioma cells; and (4) alternative processing of max mRNA in some glioma cells results in delta Max encoding mRNAs not seen in normal fetal brain.
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影响因子：
5.3
作者：
KAYE, F;BATTEY, J;MINNA, J
通讯作者：
MINNA, J
影响因子：
4.2
作者：
LAROCCA, RV;ROSENBLUM, M;ISRAEL, MA
通讯作者：
ISRAEL, MA
DOI：
10.1097/00005072-198805000-00001
发表时间：
1988-05-01
影响因子：
3.2
作者：
BIGNER, SH;BURGER, PC;BIGNER, DD
通讯作者：
BIGNER, DD
影响因子：
56.9
作者：
MAKELA, TP;KOSKINEN, PJ;ALITALO, K
通讯作者：
ALITALO, K
影响因子：
64.5
作者：
PRENDERGAST, GC;LAWE, D;ZIFF, EB
通讯作者：
ZIFF, EB