Aberrant T cell subsets and cytokines expression profile in systemic lupus erythematosus
Aberrant T cell subsets and cytokines expression profile in systemic lupus erythematosus
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系统性红斑狼疮中异常的 T 细胞亚群和细胞因子表达谱
DOI：
10.1007/s10067-018-4124-0
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发表时间：
2018-05
期刊：
影响因子：
--
通讯作者：
Joshua Liao
中科院分区：
文献类型：
--
作者：
Haiyan Zhou;Bailong Hu;Niwen Huang;Xiangang Mo;Wei Li;Bei Zhang;Bo Wei;Mingzhu Gao;Yiming Wang;Xingde Liu;Joshua Liao
探讨SLE患者T细胞亚群、趋化因子和细胞因子水平与疾病活动性和器官受累的关系。分析来自SLE患者（n= 24）和健康对照（n= 36）的血液样品。计数循环滤泡辅助T细胞（Tfh）、中枢记忆T细胞（Tcm）、效应记忆T细胞（Tem）和初始T细胞亚群的频率，并通过流式细胞术检测其表面标志物诱导T细胞共刺激因子（ICOS）和程序性死亡1（PD-1）蛋白的表达。采用系统性红斑狼疮疾病活动指数（SLEDAI）评价SLE患者的疾病状态。测定自身抗体、血清C反应蛋白（CRP）、红细胞沉降率（ESR）、IgG、补体3、补体4、细胞因子和趋化因子（如IL-21、IL-17 A和IL-1β）的浓度。Tfh和Tcm细胞亚群频率明显低于健康对照组。SLE患者PBMC中PD 1 +ICOS+Tfh、PD 1 +ICOS+Tcm和PD 1 +ICOS+Tem的比例均高于健康对照组。SLE患者血清IL-1β、IL-4、IL-6、MCP-1、IL-21和IL-17 A水平高于健康对照组。此外，免疫性血小板减少症患者的血清IL-10、IL-17 A和IL-1β比例升高。SLE患者存在T细胞亚群和细胞因子表达的异常。PD 1 +ICOS+Tem细胞亚群明显受疾病活动性的影响，免疫性血小板减少症患者血清IL-10、IL-17 A和IL-1β水平显著升高。因此，PD 1 +ICOS+Tem细胞可作为SLE患者免疫性血小板减少的重要识别工具，血清IL-10、IL-17 A和IL-1β可作为SLE患者免疫性血小板减少的有效监测指标。
To assess T cell subsets and levels of chemokines and cytokines in patients with SLE and determine their relationships between disease activity and organ involvement. Blood samples from SLE patients (n= 24) and healthy controls (n= 36) were analyzed. Frequency of circulating follicular help T cells (Tfh), central memory T cells (Tcm), effector memory T cells (Tem), and naïve T cell subsets was enumerated and their surface markers expression of inducible T cell co-stimulator (ICOS) and programmed death 1(PD-1) protein was examined by flow cytometry. The disease state in SLE patients was evaluated using the SLE Disease Activity Index (SLEDAI). Concentrations of autoantibodies, serum C-reactive protein (CRP), the erythrocyte sedimentation rate (ESR), lgG, complement 3, complement 4, cytokines, and chemokines, such as IL-21, IL-17A, and IL-1β, were measured. The frequencies of circulating Tfh and Tcm cell subsets were significantly lower than those in healthy controls. However, the percentages of circulating PD1+ICOS+Tfh, PD1+ICOS+Tcm, and PD1+ICOS+Tem of PBMCs from SLE patients were higher than those in healthy controls. Furthermore, increased levels of serum IL-1β, IL-4, IL-6, MCP-1, IL-21, and IL-17A were detected in the patients with SLE compared to healthy controls. In addition, patients with immune thrombocytopenia displayed elevated proportions of serum IL-10, IL-17A, and IL-1β. Aberrant T cell subsets and cytokines expression profile were observed in SLE patients. PD1+ICOS+Tem cell subset was clearly influenced by disease activity and serum IL-10, IL-17A, and IL-1β were significantly increased in patients with immune thrombocytopenia. Therefore, PD1+ICOS+Tem cells might serve as an important tool for recognition and serum IL-10, IL-17A, and IL-1β might be an effective monitor for SLE patients with immune thrombocytopenia.
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DOI：
10.1038/nri3405
发表时间：
2013-04
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
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影响因子：
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作者：
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发表时间：
2015
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影响因子：
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作者：
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影响因子：
4.4
作者：
Kasagi, Shimpei;Kawano, Seiji;Kumagai, Shunichi
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系统性红斑狼疮循环滤泡辅助性T细胞中Bcl-6的表达与疾病活动度呈正相关
影响因子：
8.6
作者：
Huang, Xin;Wu, Haijing;Lu, Qianjin
通讯作者：
Lu, Qianjin