A three-dimensional scaffold with precise micro-architecture and surface micro-textures.
A three-dimensional scaffold with precise micro-architecture and surface micro-textures.
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DOI：
10.1016/j.biomaterials.2009.05.023
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发表时间：
2009-09
期刊：
影响因子：
14
通讯作者：
Roy, Shuvo
中科院分区：
文献类型：
--
作者：
Mata, Alvaro;Kim, Eun Jung;Boehm, Cynthia A.;Fleischman, Aaron J.;Muschler, George F.;Roy, Shuvo
关键词：
三维（3D）结构包括精确限定的微结构和表面微纹理，被设计为向细胞和组织呈现特定的物理线索，可以在各种组织工程和再生医学应用中提供有效的支架。我们报告了一种基于微加工和软光刻的制造技术，该技术允许开发具有精确工程结构和定制表面形貌的3D支架。支架制造技术由三个关键步骤组成，首先是使用环氧基光致抗蚀剂（SU-8）进行模具的微制造，然后使用机械夹具进行精确运动控制，对单层聚二甲基硅氧烷（PDMS）进行双面成型;最后，对齐，堆叠和粘合多个PDMS层以实现3D结构。该技术用于生产3D纹理和3D光滑PDMS支架，其中表面形貌分别包括10 μ m直径/高度的柱和光滑表面。3D微制造支架的潜在效用和表面形貌的作用，随后在体外研究了成人干细胞及其所得祖细胞的组合异质群体，统称为结缔组织祖细胞（CTPs），在促进成骨细胞表型的条件下。对在3D纹理支架上培养9天的骨髓源性CTP的检查显示，与3D光滑支架上的细胞相比，细胞在三维中生长并增加细胞数量。此外，碱性磷酸酶mRNA的表达在3D纹理支架上更高，而骨钙素mRNA的表达在两种类型的支架上相当。
A three-dimensional (3D) structure comprising precisely defined microarchitecture and surface micro-textures, designed to present specific physical cues to cells and tissues, may provide an efficient scaffold in a variety of tissue engineering and regenerative medicine applications. We report a fabrication technique based on microfabrication and soft lithography that permits for the development of 3D scaffolds with both precisely engineered architecture and tailored surface topography. The scaffold fabrication technique consists of three key steps starting with microfabrication of a mold using an epoxy-based photoresist (SU-8), followed by dual-sided molding of a single layer of polydimethylsiloxane (PDMS) using a mechanical jig for precise motion control; and finally, alignment, stacking, and adhesion of multiple PDMS layers to achieve a 3D structure. This technique was used to produce 3D Texture and 3D Smooth PDMS scaffolds, where the surface topography comprised 10 μm-diameter/height posts and smooth surfaces, respectively. The potential utility of the 3D microfabricated scaffolds, and the role of surface topography, were subsequently investigated in vitro with a combined heterogeneous population of adult human stem cells and their resultant progenitor cells, collectively termed connective tissue progenitors (CTPs), under conditions promoting the osteoblastic phenotype. Examination of bone-marrow derived CTPs cultured on the 3D Texture scaffold for 9 days revealed cell growth in three dimensions and increased cell numbers compared to those on the 3D Smooth scaffold. Furthermore, expression of alkaline phosphatase mRNA was higher on the 3D Texture scaffold, while osteocalcin mRNA expression was comparable for both types of scaffolds.
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DOI：
10.1023/a:1018591724140
发表时间：
1997-04-01
影响因子：
3.7
作者：
Liu, DM
通讯作者：
Liu, DM
影响因子：
14
作者：
Borden, M;Attawia, M;Laurencin, CT
通讯作者：
Laurencin, CT
DOI：
10.1023/a:1011203226053
发表时间：
2001-01-01
影响因子：
3.7
作者：
Chu, TMG;Halloran, JW;Feinberg, SE
通讯作者：
Feinberg, SE
DOI：
10.2106/00004623-199711000-00012
发表时间：
1997-11-01
影响因子：
5.3
作者：
Muschler, GF;Boehm, C;Easley, K
通讯作者：
Easley, K
影响因子：
14
作者：
Hutmacher, DW
通讯作者：
Hutmacher, DW