The permeability of single capillaries to potassium ions.
The permeability of single capillaries to potassium ions.
复制标题
单个毛细血管对钾离子的渗透性。
DOI：
10.1085/jgp.71.2.195
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发表时间：
1978-02
期刊：
影响因子：
--
通讯作者：
Christensen O
中科院分区：
文献类型：
--
作者：
Crone C;Frøkjaer-Jensen J;Friedman JJ;Christensen O
本文报道了蛙肠系膜单个毛细血管对钾离子扩散通透性的测定方法。通过微量移液器，将注射液或输注液输送到单个毛细管中。随后的浓度变化和周围的毛细管与K（+）敏感的微电极。理论分析提供了一个定量的参考框架，用于评估所观察到的时间-浓度曲线的毛细管渗透率。单毛细管研究的优点是，通过扩散发生的表面积是已知的，是跨毛细管膜的浓度差。三种不同的技术是：（a）“单次注射”法，代表指示剂扩散技术的应用，其中将高K（+）推注注入单个毛细血管;（B）“袋”法，在毛细血管内K（+）浓度短暂增加后，测定K（+）从闭塞的毛细血管段内和紧接的毛细血管段外消失的速率;以及（c）“间隙扩散”方法，其记录在毛细血管内K（+）浓度阶跃变化之后K（+）在间隙空间中的时间和空间分布。该方法在室温下测得毛细血管膜的平均钾渗透率为67 × 10 ~（-5）cm·s ~（-1）（SD：23，n=26）。 这些数字明显高于以前报告的哺乳动物毛细血管研究使用整个器官技术。根据Pappenheimer孔模型，毛细管渗透率的这种估计与厚度为1.0 μm的膜的行为一致，该膜具有半径为110 A的等效孔，孔面积分数为0.3%，孔密度为8 μm（-2）。
This paper reports a description of methods for determining the diffusional permeability to potassium ions of single capillaries in the frog mesentery. By means of micropipettes, injections or infusions were delivered into a single capillary. The subsequent concentration variations in and about the capillary were followed with K(+)-sensitive microelectrodes. A theoretical analysis is provided which give a quantitative frame of reference for evaluating the observed time-concentration curves in terms of capillary permeability. The advantage of single capillary studies is that the surface area through which diffusion occurs is known as is the concentration difference across the capillary membrane. Three different techniques are: (a) the “single injection” method which represents an application of the indicator diffusion technique where a high-K(+) bolus is injected into a single capillary; (b) the “sack” method which determines the rate of K(+) disappearance from within and immediately outside an occluded capillary segment, after a brief increase in intracapillary K(+) concentration; and (c) the “interstitial diffusion” method which records time and spatial distribution of K(+) in the interstitial space after a step-change in intracapillary K(+) concentration. The methods gave an average potassium permeability of the capillary membrane of 67x10(-5) cm s(-1) (SD: 23, n=26) at room temperature. These figures are clearly higher than those previously reported in mammalian capillary studies using whole-organ techniques. In terms of the pappenheimer pore model, this estimate of capillary permeability is consistent with the behavior of a membrane with a thickness of 1.0 μm which possesses equivalent pores with a radius of 110 A, a fractional pore area of 0.3 percent, and a pore density of 8 μm(-2).
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影响因子：
5.5
作者：
CURRY, FE;MASON, JC;MICHEL, CC
通讯作者：
MICHEL, CC
DOI：
10.1111/j.1748-1716.1977.tb05967.x
发表时间：
1977-01-01
期刊：
ACTA PHYSIOLOGICA SCANDINAVICA
影响因子：
--
作者：
PAASKE, WP
通讯作者：
PAASKE, WP
影响因子：
3.1
作者：
PERL, W
通讯作者：
PERL, W
DOI：
10.1111/j.1748-1716.1963.tb02652.x
发表时间：
1963-01-01
期刊：
ACTA PHYSIOLOGICA SCANDINAVICA
影响因子：
--
作者：
CRONE, C
通讯作者：
CRONE, C
影响因子：
--
作者：
Landis, EM
通讯作者：
Landis, EM