ASL16 gene, a member of AS2/LOB family, is essential for lateral root formation in Arabidopsis
ASL16 gene, a member of AS2/LOB family, is essential for lateral root formation in Arabidopsis
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ASL16 基因是 AS2/LOB 家族的成员，对于拟南芥侧根的形成至关重要
DOI：
10.1515/biolog-2016-0120
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发表时间：
2016
期刊：
影响因子：
1.5
通讯作者：
Jihong Jiang
中科院分区：
文献类型：
--
作者：
Laisheng Meng;Bizhu He;Xiaoying Cao;Xuqin Yang;Jiangping Song;Yibo Wang;Jihong Jiang
拟南芥的ASYMMETRIC LEAVES 2-LIKE 16基因是ASYMMETRIC LEAVES 2的成员。(AS2)/外侧器官边界（LOB）结构域基因家族。目前的报道表明，在缺乏ARF 7和ARF 19的情况下，ASL 16的过表达诱导侧根形成;而在ARF 7和ARF 19双敲除突变体中，侧根形成严重减少。反义mRNA已被证明是抑制植物中特定基因表达的有用工具;更重要的是，它是特异性的，因为只有所需蛋白质的mRNA水平被改变，而基因家族的其他成员不受影响。为了进一步研究ASL 16基因在侧根生长发育中的作用，我们将ASL 16基因的反义表达载体导入拟南芥中。（Col-0）;并获得了一些35 S：ASL 16拟南芥植物。在萌发后11天（DAG），野生型幼苗的许多侧根发育良好，而反义35 S：ASL 16幼苗的侧根很少。然而，在第5、8和16 DAG，这些转基因幼苗中的侧根与野生型植物的侧根不明显。一系列的数据表明，这些反义35 S：ASL 16植物的主根形成没有受到影响，但它们的侧根形成受到严重抑制。
ASYMMETRIC LEAVES2-LIKE16 gene of Arabidopsis is a member of the ASYMMETRIC LEAVES2.(AS2)/LATERAL ORGAN BOUNDARIES (LOB) domain gene family. Current reports have indicated that overexpression.of ASL16 induces lateral root formation in the absence of ARF7 and ARF19; whereas lateral root formation is severely.reduced in the arf 7 and arf 19 double knockout mutants. Antisense mRNA has testified to be a useful tool to inhibit the.expression of specific genes in plants; more significantly, it is specific in the sense that only the mRNA level of the desired.protein being changed, while other members of the gene family remain unaffected. To further explore ASL16 function in.lateral root growth and development, we introduced an antisense-expression construct of the ASL16 gene to Arabidopsis.(Col-0); and acquired some 35S:ASL16 Arabidopsis plants. With 11 day after germination (DAG), many lateral roots in.wild-type seedlings were well developed, whereas a few lateral roots in these antisense 35S:ASL16 seedlings were produced..However, with 5, 8 and 16 DAG, the lateral roots in these transgenic seedlings were not distinct with those of wild-type.plants. A series of data indicate that primary root formation of these antisense 35S:ASL16 plants was not affected; whereas.their lateral root formation was severely inhibited.
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影响因子：
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