Anti-VEGF-R2 Aptamer and RGD Peptide Synergize in a Bifunctional Hydrogel for Enhanced Angiogenic Potential.
Anti-VEGF-R2 Aptamer and RGD Peptide Synergize in a Bifunctional Hydrogel for Enhanced Angiogenic Potential.
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抗VEGF-R2适体和RGD肽在双功能水凝胶中协同作用以增强血管生成潜力。
DOI：
10.1002/mabi.202000337
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发表时间：
2021-03
影响因子：
4.6
通讯作者：
Allen JB
中科院分区：
文献类型：
--
作者：
Roy T;James BD;Allen JB
水凝胶由于其吸收和溶胀性质以及其模拟天然细胞外基质的能力而获得了用于组织再生和伤口愈合的兴趣。它们在伤口愈合中的用途具体地可以是贴剂或伤口敷料的形式，或者它们可以作为填料、原位凝胶在伤口床内施用以促进愈合。巯基化透明质酸-聚乙烯二丙烯酸酯（tHA-PEGDA）水凝胶由于其在生理温度和pH下的短凝胶化时间而理想地用于此目的。但是这些水凝胶单独是不够的，并且需要添加组分以获得生物活性。在这项工作中，将RGD粘附肽和抗血管内皮生长因子受体-2（VEGF-R2）DNA适体掺入tHA-PEGDA水凝胶中以制备双功能透明质酸水凝胶。RGD肽促进细胞的附着和生长，而抗VEGF-R2 DNA适体似乎提高细胞活力，诱导细胞迁移，并通过内皮细胞形成管刺激血管生成的开始。这种双功能水凝胶支持细胞培养并具有改善的生物学特性。数据表明，这些水凝胶可用于先进的组织再生应用，如伤口愈合。在这项工作中，双功能透明质酸基水凝胶支持内皮细胞培养，并显示出改善的生物学特性。凝胶用RGD肽和抗VEGF-R2 DNA适体两者官能化。数据表明，通过内皮细胞的管形成，改善了细胞活力、细胞迁移和启动血管生成。数据表明，这些水凝胶可用于伤口愈合。
Hydrogels have gained interest for use in tissue regeneration and wound healing because of their absorbing and swelling properties as well as their ability to mimic the natural extracellular matrix. Their use in wound healing specifically may be in the form of a patch or wound dressing or they may be administered within the wound bed as a filler, gel in situ, to promote healing. Thiolated hyaluronic acid-polyethylene diacrylate (tHA-PEGDA) hydrogels are ideal for this purpose due to their short gelation times at physiological temperature and pH. But these hydrogels alone are not enough and require added components to gain bioactivity. In this work, RGD adhesion peptides and an anti-vascular endothelial growth factor receptor-2 (VEGF-R2) DNA aptamer are incorporated into a tHA-PEGDA hydrogel to make a bifunctional hyaluronic acid hydrogel. RGD peptides promote attachment and growth of cells while the anti-VEGF-R2 DNA aptamer seems to improve cell viability, induce cell migration, and spur the onset of angiogenesis by tube formation by endothelial cells. This bifunctional hydrogel supports cell culture and has improved biological properties. The data suggests that these hydrogels can be used for advanced tissue regeneration applications such as in wound healing. In this work, a bifunctional hyaluronic acid-based hydrogel supports endothelial cells culture and shows improved biological properties. The gel is functionalized with both RGD peptides and anti-VEGF-R2 DNA aptamer. The data suggests improved cell viability, cell migration, and the initiation angiogenesis by tube formation by endothelial cells. The data suggests that these hydrogels can be used in wound healing.
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