ankAT-1 is a novel gene mediating the apical tuft formation in the sea urchin embryo.
ankAT-1 is a novel gene mediating the apical tuft formation in the sea urchin embryo.
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DOI：
10.1016/j.ydbio.2010.09.011
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发表时间：
2010-12-01
影响因子：
2.7
通讯作者：
Inaba K
中科院分区：
文献类型：
--
作者：
Yaguchi S;Yaguchi J;Wei Z;Shiba K;Angerer LM;Inaba K
在海胆胚胎中，顶端簇形成于神经源性动物板内。 FoxQ2（早期囊胚阶段在动物板中特异性表达的最早因子之一）被敲除时，顶端簇的结构发生改变。为了确定这种表型的基础，我们使用微阵列分析鉴定了 FoxQ2 依赖性基因。 FoxQ2 变形体中下调最强烈的基因编码其 N 末端结构域具有锚蛋白重复区域的蛋白质。我们将该基因命名为 ankAT-1，即顶端簇特有的含锚蛋白的基因。最初，其在极早期囊胚阶段胚胎的动物极区域中的表达是FoxQ2独立的，但从间充质囊胚阶段开始变得依赖于FoxQ2，并在3日幼虫的动物板中持续。此外，与 FoxQ2 一样，该基因在缺乏核 β-连环蛋白的胚胎中形成的扩展的顶端簇区域中表达。当通过注射吗啡啉敲除AnkAT-1时，所得胚胎中动物板上的纤毛要短得多，其运动性也低于其他外胚层细胞中的运动性纤毛，并且与长顶端簇状纤毛相似。我们得出结论，AnkAT-1 参与调节顶端簇纤毛的长度。
In sea urchin embryos, the apical tuft forms within the neurogenic animal plate. When FoxQ2, one of the earliest factors expressed specifically in the animal plate by early blastula stage, is knocked down, the structure of the apical tuft is altered. To determine the basis of this phenotype, we identified FoxQ2-dependent genes using microarray analysis. The most strongly down-regulated gene in FoxQ2 morphants encodes a protein with ankyrin repeats region in its N-terminal domain. We named this gene ankAT-1, Ankyrin-containing gene specific for Apical Tuft. Initially its expression in the animal pole region of very early blastula stage embryos is FoxQ2-independent but becomes FoxQ2-dependent beginning at mesenchyme blastula stage and continuing in the animal plate of 3-day larvae. Furthermore, like FoxQ2, this gene is expressed throughout the expanded apical tuft region that forms in embryos lacking nuclear β-catenin. When AnkAT-1 is knocked-down by injecting a morpholino, the cilia at the animal plate in the resulting embryos are much shorter and their motility is less than that of motile cilia in other ectoderm cells, and remains similar to that of long apical tuft cilia. We conclude that AnkAT-1 is involved in regulating the length of apical tuft cilia.
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影响因子：
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通讯作者：
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