Functional studies of per os infectivity factors of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus.
Functional studies of per os infectivity factors of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus.
复制标题
棉铃虫单核衣壳核多角体病毒经口感染因子的功能研究。
DOI：
10.1099/vir.0.2008/002352-0
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发表时间：
2008-09
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
本研究对棉铃虫单核衣壳核多角体病毒（HearNPV）的四种经口感染因子（PIFs）进行了联合功能研究。构建了缺失p74（Ha20）、pif1（Ha111）、pif2（Ha1……（此处原文似乎不完整）的HearNPV杆粒。
A combined functional investigation on the four per os infectivity factors (PIFs) of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HearNPV) was conducted in this study. HearNPV bacmids with deletions of p74 (Ha20), pif1 (Ha111), pif2 (Ha132) and pif3 (Ha98) were constructed individually by homologous recombination in Escherichia coli cells. Repaired bacmids with respective pifs were also constructed. Western blot analyses revealed that all four PIFs were structural components of the envelope of HearNPV occlusion-derived virus (ODV). Electron microscopy showed that deletion of the pifs did not have any obvious effects on the morphology of the occlusion bodies (OBs). Bioassay analyses indicated that deletion of any of the above pifs resulted in loss of oral infectivity of OBs. The mixtures of the four pif-deletion mutants also resulted in deficiency of oral infectivity, implying that the four PIFs must be structural components of the same ODV to accomplish their function. Repairing of the respective genes into the pif-deletion bacmids could rescue the oral infectivity of the pif-deletion viruses. Calcofluor, which can damage the peritrophic membrane (PM), could not rescue the defects of the oral infectivity of the pif-deletion viruses, indicating that the PM is not likely to be the functional target of the PIFs.
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DOI：
10.1099/0022-1317-82-9-2279
发表时间：
2001-09
期刊：
The Journal of general virology
影响因子：
--
作者：
J. Slack;Edward M. Dougherty;S. Lawrence
通讯作者：
J. Slack;Edward M. Dougherty;S. Lawrence
影响因子：
5.5
作者：
Xiulian Sun;G. Zhang;Z. Zhang;Zhìhóng Hú;J. Vlak;B. Arif
通讯作者：
Xiulian Sun;G. Zhang;Z. Zhang;Zhìhóng Hú;J. Vlak;B. Arif
影响因子：
5.4
作者：
M. Summers;G. Smith
通讯作者：
M. Summers;G. Smith
影响因子：
--
作者：
Slack J;Arif BM
通讯作者：
Arif BM
影响因子：
3.8
作者：
Wang, P;Granados, RR
通讯作者：
Granados, RR