Effects of Jin-Ying-Tang on Staphylococcus aureus-induced mastitis in rabbit
Effects of Jin-Ying-Tang on Staphylococcus aureus-induced mastitis in rabbit
复制标题
金英汤对金黄色葡萄球菌所致家兔乳腺炎的影响
DOI：
10.3109/08923973.2012.655423
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发表时间：
2012-09
影响因子：
3.3
通讯作者：
Yi, Qiong
中科院分区：
文献类型：
--
作者：
Wang, Lu;He, Chang-Liang;He, Bing-Kun;Guo, Qing;Xiao, Chun-Gang;Yi, Qiong
本研究观察了由当归、蒲公英、蒲公英、大黄、黄芪、当归组成的中药复方金英汤对金黄色葡萄球菌所致家兔乳腺炎的治疗作用。用1.5 × 107菌落形成单位（CFU）的S.金黄色葡萄球菌的第三对乳头基部，它们被处理和预处理JYT，以检测配方效应。结果表明，JYT能降低家兔葡萄球菌性乳腺炎的发生率。为了进一步研究JYT的作用机制，我们检测了血液和感染组织中白细胞计数和炎症介质如TNF-α和IL-6的水平。从组织学研究和血液分析中发现，JYT可以抑制感染乳腺组织中的白细胞浸润，并显著抑制血液中白细胞总数和淋巴细胞（LYM）、单核细胞（MON）和粒细胞（GRA）的百分比。酶联免疫吸附试验（ELISA）结果显示，JYT能显著降低血清和乳腺组织中TNF-α和IL-6的含量。这些数据的分析表明，JYT有效地抑制炎症反应，以减少乳腺炎的发生在兔模型。
The present study was performed to investigate the effects of Jin-Ying-Tang (JYT), a Chinese herbal formula containing Lonicera japonica, Herba taraxaci, Fructus trichosanthis, Fructus forsythia, Radix et rhizoma rhei, Astragalus membranaceus, Angelica sinensis, on rabbit mastitis induced by Staphylococcus aureus. Suckling rabbits were challenged with 1.5 × 107 colony forming unit (CFU) of S. aureus at the base of the third pair teats, and they were treated and pretreated with JYT to detect the formula effects. The results showed that JYT could reduce the occurrence of Staphylococcal mastitis in rabbit model. To further investigate the action mechanism of JYT, we examined the leukocyte counts and inflammatory mediator levels such as TNF-α and IL-6 in blood and infected tissue. From histological study and blood analysis, we found that JYT could suppress leukocyte infiltration in infected mammary gland tissue and significantly inhibit the total leukocyte counts and lymphocytes (LYM), monocytes (MON) and granulocytes (GRA) fractions of leukocyte counts in blood. Enzyme-linked immunosorbent assay (ELISA) results showed JYT significantly decreased the TNF-α and IL-6 concentrations in serum and mammary gland. The analysis of these data suggested that JYT effectively inhibited inflammatory responses to reduce the occurrence of mastitis in rabbit model.
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DOI：
--
发表时间：
2007
期刊：
--
影响因子：
--
作者：
O. Radostits;S. Done
通讯作者：
O. Radostits;S. Done
影响因子：
--
作者：
T. Kuroishi;K. Komine;K. Asai;J. Kobayashi;Kouichi Watanabe;Takahiro Yamaguchi;S. Kamata;K. Kumagai
通讯作者：
T. Kuroishi;K. Komine;K. Asai;J. Kobayashi;Kouichi Watanabe;Takahiro Yamaguchi;S. Kamata;K. Kumagai
影响因子：
3.5
作者：
Y. H. Park;Lawrence K. Fox;M. Hamilton;William C. Davis
通讯作者：
Y. H. Park;Lawrence K. Fox;M. Hamilton;William C. Davis
影响因子：
3.5
作者：
Dingwell, RT;Leslie, KE;Bagg, R
通讯作者：
Bagg, R
影响因子：
5.4
作者：
Shen, Hua-Hao;Wang, Kai;Huang, Hua-Qiong
通讯作者：
Huang, Hua-Qiong