High-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictus.
High-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictus.
复制标题
DOI：
10.3390/v12111343
复制
发表时间：
2020-11-23
期刊：
影响因子：
--
通讯作者：
Alto BW
中科院分区：
文献类型：
--
作者：
Honório NA;Câmara DCP;Wiggins K;Eastmond B;Alto BW
病媒能力是指病媒获得、维持和传播病原体的能力。在中等填充的毛细管中收集蚊子唾液已经成为近似虫媒病毒传播的标准。然而，这种方法是耗时和劳动密集型的。在这里，我们比较了毛细管方法的替代高通量检测方法，收集唾液的纸卡上饱和蜂蜜，（FTA卡）和（滤纸）试剂用于保存核酸的埃及伊蚊和白纹伊蚊感染的基孔肯雅病毒（CHIKV）的两个新兴的基因型。模型结果表明，亚洲基因型CHIKV传播在两个Ae收获腿。埃及伊蚊和埃及伊蚊。白纹伊蚊增加了具有阳性唾液感染和较高唾液病毒滴度的女性的几率，而对于IOL基因型，仅对Ae观察到相同的效果。埃及人。在三种检测方法中，FTA卡在检测Ae感染唾液方面的效果明显更好。埃及伊蚊和埃及伊蚊。白纹伊蚊雌蚊的检测效果优于毛细管法和滤纸法，对于亚洲型的检测效果与毛细管法相同。我们没有发现检测方法在检测亚洲和IOL基因型的较高病毒滴度方面的显著影响。我们的研究结果进行了讨论，根据不同的测试检测方法的局限性。
Vector competence refers to the ability of a vector to acquire, maintain, and transmit a pathogen. Collecting mosquito saliva in medium-filled capillary tubes has become the standard for approximating arbovirus transmission. However, this method is time-consuming and labor-intensive. Here we compare the capillary tube method to an alternative high-throughput detection method the collection of saliva on paper cards saturated with honey, with (FTA card) and without (filter paper) reagents for the preservation of nucleic acid for Aedes aegypti and Aedes albopictus mosquitoes infected with two emerging genotypes of the chikungunya virus (CHIKV). Model results showed that the Asian genotype CHIKV dissemination in the harvested legs of both Ae. aegypti and Ae. albopictus increased the odds of females having a positive salivary infection and higher salivary viral titers, while for the IOL genotype the same effect was observed only for Ae. aegypti. Of the three tested detection methods, the FTA card was significantly more effective at detecting infected saliva of Ae. aegypti and Ae. albopictus females than the capillary tube and filter paper was as effective as the capillary tube for the Asian genotype. We did not find significant effects of the detection method in detecting higher viral titer for both Asian and IOL genotypes. Our results are discussed in light of the limitations of the different tested detection methods.
登录
查看更多内容
影响因子：
3.8
作者：
Faye O;Freire CC;Iamarino A;Faye O;de Oliveira JV;Diallo M;Zanotto PM;Sall AA
通讯作者：
Sall AA
DOI：
10.4269/ajtmh.1966.15.989
发表时间：
1966-01-01
影响因子：
3.3
作者：
HURLBUT, HS
通讯作者：
HURLBUT, HS
DOI：
10.3390/v6114294
发表时间：
2014-11-11
期刊：
Viruses
影响因子：
--
作者：
Jupatanakul N;Sim S;Dimopoulos G
通讯作者：
Dimopoulos G
影响因子：
3.8
作者：
Franz AW;Sanchez-Vargas I;Raban RR;Black WC 4th;James AA;Olson KE
通讯作者：
Olson KE
影响因子：
2.1
作者：
Alto, Barry W.;Connelly, C. Roxanne;Karr, Nicholas
通讯作者：
Karr, Nicholas