Bmp suppression in mangrove killifish embryos causes a split in the body axis.
Bmp suppression in mangrove killifish embryos causes a split in the body axis.
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DOI：
10.1371/journal.pone.0084786
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Kudoh T
中科院分区：
文献类型：
--
作者：
Mourabit S;Moles MW;Smith E;van Aerle R;Kudoh T
骨形态发生蛋白（Bmp）是脊椎动物体平面形成的主要参与者，因为它们在背腹（DV）轴的模式化中起关键作用。尽管Bmp信号在脊椎动物中具有高度保守的性质，但改变该途径的后果可能具有物种特异性。在这里，我们报告，BMP起着重要的作用，在胚胎的外包，卵黄合胞体层（YSL）的运动，和前后（AP）轴的形成在自我繁殖的红树林鱼，Kryptolebias marmoratus。阶段和剂量特定的暴露胚胎的BMP抑制剂dorsomorphin（DM）产生三种不同的形态，最极端的条件下创建分裂体表型，其特征在于一个极短的AP轴的神经管，体节，和脊索双边分裂。此外，部分尾神经组织与主体分离，并在胚胎后部形成细胞岛。这种裂体表型在其他动物中尚未报道，表明BMP的修饰可能导致其他脊椎动物物种发育过程中显着不同的后果。
Bone morphogenetic proteins (Bmp) are major players in the formation of the vertebrate body plan due to their crucial role in patterning of the dorsal-ventral (DV) axis. Despite the highly conserved nature of Bmp signalling in vertebrates, the consequences of changing this pathway can be species-specific. Here, we report that Bmp plays an important role in epiboly, yolk syncytial layer (YSL) movements, and anterior-posterior (AP) axis formation in embryos of the self-fertilizing mangrove killifish, Kryptolebias marmoratus. Stage and dose specific exposures of embryos to the Bmp inhibitor dorsomorphin (DM) produced three distinctive morphologies, with the most extreme condition creating the splitbody phenotype, characterised by an extremely short AP axis where the neural tube, somites, and notochord were bilaterally split. In addition, parts of caudal neural tissues were separated from the main body and formed cell islands in the posterior region of the embryo. This splitbody phenotype, which has not been reported in other animals, shows that modification of Bmp may lead to significantly different consequences during development in other vertebrate species.
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