RPN1a, a subunit of the 26S proteasome, controls trichome development in Arabidopsis.
RPN1a, a subunit of the 26S proteasome, controls trichome development in Arabidopsis.
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RPN1a 是 26S 蛋白酶体的一个亚基，控制拟南芥中毛状体的发育。
DOI：
10.1016/j.plaphy.2015.01.012
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发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Liu Xuanming
中科院分区：
文献类型：
--
作者：
Yu Dashi;Yu Feng;Du Changqing;Li Xiushan;Zhao Xiaoying;Liu Xuanming
泛素介导的26S蛋白酶体途径（UPS）对植物生长发育具有重要意义。此前的研究表明，26S蛋白酶体的一个亚基，名为RPN1a，参与拟南芥毛状体的分支。 RPN1 突变会在叶子上产生更多的毛状分支。在这里，我们发现与野生型植物相比，RPN1中的T-DNA插入突变导致主茎上的毛状体分支以及莲座叶和主茎上的毛状体数量增加。表达分析结果表明，Therpn1突变体中促进毛状体起始的ZFP6、ZFP5、GIS、GL1、GL2、GL3、TTG1和MYB23的转录水平上调，负责毛状体分支增加的FRC4的表达在therpn1a突变体中也增加。此外，GA（赤霉素）和CK（细胞分裂素）处理显着抑制RPN1的mRNA表达水平，这两种重要的植物激素在毛状体发育中发挥重要作用。这些结果表明 RPN1a 可能通过 GA 和 CK 信号通路参与毛状体发育。
The ubiquitin-mediated 26S proteasome pathway (UPS) is of great importance to plant growth and development. Previously research showed that a subunit of the 26S proteasome, named RPN1a, was involved in trichome's branching in Arabidopsis. Mutation inRPN1agive rise to more trichome branches on leaves. Here, we found that T-DNA insertion mutation inRPN1aresulted in increased trichome branches on main stem, and trichome number on rosette leaves and the main stem compared with the wild type plant. Expression analysis results showed that the transcription levels ofZFP6,ZFP5,GIS,GL1,GL2,GL3,TTG1andMYB23, which promote trichome initiation, were up-regulated in therpn1amutant, and expression ofFRC4, which is responsible for increased trichome branching, was also increased in therpn1amutant. Moreover, the mRNA expression level ofRPN1awas significantly repressed by GA (gibberellin) and CK (cytokinin) treatment, which are two important phytohormones that play essential roles in trichome development. These results demonstrate that RPN1a may be involved in trichome development through the GA and CK signaling pathways.
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