Morphological identification and development of neurite in Drosophila ventral nerve cord neuropil.
Morphological identification and development of neurite in Drosophila ventral nerve cord neuropil.
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果蝇腹神经索神经纤维神经突的形态学鉴定和发育
DOI：
10.1371/journal.pone.0105497
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Xie W
中科院分区：
文献类型：
--
作者：
Gan G;Lv H;Xie W
在果蝇中，腹神经索（VNC）占据了幼虫中枢神经系统（CNS）的大部分。然而，很少有文献详细阐述果蝇幼虫 VNC 神经毡中神经突的具体类型和生长（根据其结构外观来定义）。在这里，我们利用透射电子显微镜报告了胚胎和幼虫阶段不同类型 VNC 神经突在十个选定时间点的超微结构发育。根据囊泡内容物的类型分类，轴突神经突可分为四种类型：透明囊泡（CV）神经突具有透明囊泡和一些 T 形结构；透明囊泡（CV）神经突具有透明囊泡和一些 T 形结构；致密核囊泡 (DV) 神经突具有致密核囊泡且无 T 形结构；混合囊泡 (MV) 神经突具有混合囊泡和一些 T 形结构；大囊泡 (LV) 神经突以大的半透明球形囊泡为主，但很少显示 T 形结构。我们发现CV神经突发生了戏剧性的重塑，可分为五个发育阶段。神经突在初级（P）期呈空泡状，在第二（S）期有线粒体、微管或大的暗囊泡，在第三（T）期含有未成熟的突触特征。随后的分叉 (B) 相似乎经历了重大重塑，出现分叉或枝晶生长。在最终成熟 (M) 阶段，高密度的相应突触小泡分布在 T 形结构周围。 CVI 神经突亚型有四种形态学阐述。首先，新的神经突在轴突末端产生。其次，新的神经突沿着轴突冒泡。第三，先前存在的神经突发芽并发育成多个神经突。最后，成束的轴突形成不规则形状的神经突。大多数M期CVI神经突的直径约为1.5-3微米，它们适合通过光学显微镜分析其形态和特定蛋白质的亚细胞定位，并且可以作为中枢神经系统体内发育的潜在模型。
In Drosophila, ventral nerve cord (VNC) occupies most of the larval central nervous system (CNS). However, there is little literature elaborating upon the specific types and growth of neurites as defined by their structural appearance in Drosophila larval VNC neuropil. Here we report the ultrastructural development of different types VNC neurites in ten selected time points in embryonic and larval stages utilizing transmission electron microscopy. There are four types of axonal neurites as classified by the type of vesicular content: clear vesicle (CV) neurites have clear vesicles and some T-bar structures; Dense-core vesicle (DV) neurites have dense-core vesicles and without T-bar structures; Mixed vesicle (MV) neurites have mixed vesicles and some T-bar structures; Large vesicle (LV) neurites are dominated by large, translucent spherical vesicles but rarely display T-bar structures. We found dramatic remodeling in CV neurites which can be divided into five developmental phases. The neurite is vacuolated in primary (P) phase, they have mitochondria, microtubules or big dark vesicles in the second (S) phase, and they contain immature synaptic features in the third (T) phase. The subsequent bifurcate (B) phase appears to undergo major remodeling with the appearance of the bifurcation or dendritic growth. In the final mature (M) phase, high density of commensurate synaptic vesicles are distributed around T-bar structures. There are four kinds of morphological elaboration of the CVI neurite sub-types. First, new neurite produces at the end of axon. Second, new neurite bubbles along the axon. Third, the preexisting neurite buds and develops into several neurites. The last, the bundled axons form irregularly shape neurites. Most CVI neurites in M phase have about 1.5–3 µm diameter, they could be suitable to analyze their morphology and subcellular localization of specific proteins by light microscopy, and they could serve as a potential model in CNS in vivo development.
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影响因子：
3.7
作者：
Hodges JL;Newell-Litwa K;Asmussen H;Vicente-Manzanares M;Horwitz AR
通讯作者：
Horwitz AR
影响因子：
2.5
作者：
Leiss, Florian;Groh, Claudia;Tavosanis, Gaia
通讯作者：
Tavosanis, Gaia
影响因子：
2.6
作者：
Beckervordersandforth, Ruth M.;Rickert, Christof;Technau, Gerhard M.
通讯作者：
Technau, Gerhard M.
影响因子：
3.3
作者：
Robert, F.;Cloix, J-F.;Hevor, T.
通讯作者：
Hevor, T.
DOI：
10.1007/bf02179499
发表时间：
1995-05-01
期刊：
ROUXS ARCHIVES OF DEVELOPMENTAL BIOLOGY
影响因子：
--
作者：
ITO, K;URBAN, J;TECHNAU, GM
通讯作者：
TECHNAU, GM