Stereotypical patterns of epileptiform calcium signal in hippocampal CA1, CA3, dentate gyrus and entorhinal cortex in freely moving mice
Stereotypical patterns of epileptiform calcium signal in hippocampal CA1, CA3, dentate gyrus and entorhinal cortex in freely moving mice
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自由活动小鼠海马 CA1、CA3、齿状回和内嗅皮层癫痫样钙信号的刻板模式
DOI：
10.1038/s41598-019-41241-x
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发表时间：
2019-03
影响因子：
4.6
通讯作者：
Shen Hui
中科院分区：
文献类型：
--
作者：
Zhang Xin;Qiao Zhihong;Liu Nannan;Gao Lili;Wei Liangpeng;Liu Aili;Ma Zengguang;Wang Feifei;Hou Shaowei;Li Jisheng;Shen Hui
癫痫是一种多病因的脑功能障碍综合征。癫痫引起的海马神经元损伤可能是导致认知障碍的原因之一，但其潜在机制仍有待进一步阐明。kainic acid （KA）模型被广泛应用于对颞叶癫痫发生机制的认识。纤维光度法是一种适用于记录自由运动动物脑深部神经元钙活性的信号检测技术。在此，我们采用基于光纤的方法，实时监测皮下注射KA后自由运动小鼠的神经元群活动。我们观察到KA给药导致海马CA1、CA3和齿状回（DG）以及内嗅皮质（EC）中一到三种典型的癫痫样钙活性模式。海马CA1区有三种波，分别为波1、波2和慢闪。CA3区和DG区均出现波1和波2，而EC区仅出现波1。在这些癫痫样钙信号中，我们观察到高振幅和长时间的钙波作为波2的一部分，类似于皮层扩张性抑制（CSD），总是在癫痫发作结束时或之后出现。由于癫痫样钙信号的相同特征出现在不同的脑区，钙信号可能不具有区域特异性，而可能表现为细胞类型特异性。从而为癫痫发病机制和癫痫样信号传递的研究提供支持。
Epilepsy is a multi-etiological brain dysfunction syndrome. Hippocampal neuronal damage induced by seizures may be one of the causes leading to cognitive impairment, but the underlying mechanism remains to be further elucidated. The kainic acid (KA) model of temporal lobe epilepsy is widely used in understanding of the epileptogenesis. Fiber photometry is a signal detection technology suitable for recording calcium activity of neurons in the deep brain of freely moving animal. Here, we used the optical fiber-based method to monitor the real-time neuronal population activities of freely moving mice after subcutaneous injection of KA. We observed that KA administration led to one to three kinds of stereotypical patterns of epileptiform calcium activity in CA1, CA3, and dentate gyrus (DG) of the hippocampus, as well as the entorhinal cortex (EC). There were three kinds of waves in the hippocampal CA1, which we named wave 1, wave 2 and slow flash. Wave 1 and wave 2 appeared in both the CA3 and DG regions, but the EC only showed wave 1. In these epileptiform calcium signals, we observed a high amplitude and long duration calcium wave as a part of wave 2, which resembled cortical spreading depression (CSD) and always appeared at or after the end of seizure. Because the same characteristic of epileptiform calcium signal appeared in different brain regions, calcium signal may not exist with region specificity, but may exhibit a cell type specific manner. Thus, our work provides a support for the pathogenesis of epilepsy and epileptiform signal transmission research.
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影响因子：
2.6
作者：
Daniel, Andy G. S.;Laffont, Philippe;Schwartz, Theodore H.
通讯作者：
Schwartz, Theodore H.
影响因子：
11.1
作者：
Xu, Zhenghao;Wang, Yi;Chen, Bin;Xu, Cenglin;Wu, Xiaohua;Wang, Ying;Zhang, Shihong;Hu, Weiwei;Wang, Shuang;Guo, Yi;Zhang, Xiangnan;Luo, Jianhong;Duan, Shumin;Chen, Zhong
通讯作者：
Chen, Zhong
影响因子：
5
作者：
Thom M
通讯作者：
Thom M
DOI：
10.1523/jneurosci.14-09-05525.1994
发表时间：
1994-09
期刊：
--
影响因子：
--
作者：
Sabine Bahn;Benedikt Volk;William Wisdenl
通讯作者：
Sabine Bahn;Benedikt Volk;William Wisdenl
DOI：
10.1093/cercor/bhv054
发表时间：
2015-11
期刊：
Cerebral cortex (New York, N.Y. : 1991)
影响因子：
--
作者：
Enger R;Tang W;Vindedal GF;Jensen V;Johannes Helm P;Sprengel R;Looger LL;Nagelhus EA
通讯作者：
Nagelhus EA