Light scattering properties vary across different regions of the adult mouse brain.
Light scattering properties vary across different regions of the adult mouse brain.
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DOI：
10.1371/journal.pone.0067626
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Klug A
中科院分区：
文献类型：
--
作者：
Al-Juboori SI;Dondzillo A;Stubblefield EA;Felsen G;Lei TC;Klug A
最近发展起来的光遗传工具为光学兴奋或抑制神经活动提供了强有力的方法。在典型的活体实验中，光通过植入的光纤传输到深核。光强随着与光纤末端距离的增加而减弱，这决定了光遗传蛋白可以被成功激活的组织体积。然而，这种有效光强度的体积是否以及如何随着大脑区域或波长的变化而变化还没有得到系统的研究。这项研究的目的是测量和比较光在小鼠大脑不同区域的散射情况。我们通过光纤将不同波长的光传输到小鼠脑干、中脑和前脑组织的急性切片上。我们测量了光强度作为距离光纤尖端的函数，并使用这些数据来模拟光在小鼠大脑特定区域的传播。我们发现不同脑区的有效衰减系数有很大的差异，这就导致了光发生实验对光强度的需求有很大的差异。不同波长的光的使用还会改变光对给定大脑区域的照明方式。我们创建了成年小鼠大脑有效衰减系数的大脑图谱，并将我们的数据整合到一个应用程序中，该应用程序可用于估计光散射以及在给定组织体积内进行光遗传操作所需的光强度。
Recently developed optogenetic tools provide powerful approaches to optically excite or inhibit neural activity. In a typical in-vivo experiment, light is delivered to deep nuclei via an implanted optical fiber. Light intensity attenuates with increasing distance from the fiber tip, determining the volume of tissue in which optogenetic proteins can successfully be activated. However, whether and how this volume of effective light intensity varies as a function of brain region or wavelength has not been systematically studied. The goal of this study was to measure and compare how light scatters in different areas of the mouse brain. We delivered different wavelengths of light via optical fibers to acute slices of mouse brainstem, midbrain and forebrain tissue. We measured light intensity as a function of distance from the fiber tip, and used the data to model the spread of light in specific regions of the mouse brain. We found substantial differences in effective attenuation coefficients among different brain areas, which lead to substantial differences in light intensity demands for optogenetic experiments. The use of light of different wavelengths additionally changes how light illuminates a given brain area. We created a brain atlas of effective attenuation coefficients of the adult mouse brain, and integrated our data into an application that can be used to estimate light scattering as well as required light intensity for optogenetic manipulation within a given volume of tissue.
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影响因子：
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